Recombinant Prosaposin antibody

Catalog No. ABIN7477571

The Mouse Monoclonal anti-Prosaposin antibody has been validated for IHC. It is suitable to detect Prosaposin in samples from Human.

Quick Overview for Recombinant Prosaposin antibody (ABIN7477571)

Target: Prosaposin (PSAP)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Prosaposin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-452M

Product Details anti-Prosaposin Antibody

Immunogen: Recombinant full-length human ACPP protein

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Prostate: A strong PSAP immunostaining should, be seen in all glandular cells.

Negative Control: Appendix: All epithelial and non-epithelial structures must not show any PSAP immunostaining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Prosaposin

Target: Prosaposin (PSAP)

Alternative Name: PSAP

Background: 5'-nucleotidase (5'-NT), Acid phosphatase prostate, ACP3, Ecto-5'-nucleotidase, Prostatic acid phosphatase (PAP), Prostatic acid phosphatase, Thiamine monophosphatase (TMPase),PSAP antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P15309

Pathways: Positive Regulation of Endopeptidase Activity