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Recombinant Synaptophysin antibody (AA 274-313)

The Rabbit Monoclonal anti-Synaptophysin antibody is suitable to detect Synaptophysin in samples from Human. It has been validated for IHC.

Cerebellum molecular granular and Purkinje layers white matter Synaptophysin is strongly expressed in cerebellum molecular Purkinje and granular layer (Recombinant Synaptophysin antibody (AA 274-313))

IHC

Thyroid Medullary carcinoma with strong cytoplasmic synaptophysin immunostaining of all tumor cells (Recombinant Synaptophysin antibody (AA 274-313))

IHC

Pancreas A strong synaptophysin immunostaining is seen in islet cells of Langerhans (Recombinant Synaptophysin antibody (AA 274-313))

IHC

3 images

Catalog No. ABIN7470669

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Quick Overview for Recombinant Synaptophysin antibody (AA 274-313) (ABIN7470669)

Target

See all Synaptophysin (SYP) Antibodies

Synaptophysin (SYP)

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Synaptophysin antibody is un-conjugated

Application

Immunohistochemistry (IHC)

Clone

462R

Discover our top product SYP Primary Antibody

Binding Specificity
15

13

9

5

5

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 274-313

Purpose

Synaptophysin antibody (462R)

Immunogen

Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotype

IgG
anti-Synaptophysin (SYP) (AA 150-250) antibody
SYP Reactivity: Human WB, IHC, IF, IP Host: Rabbit Polyclonal unconjugated

anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibody
SYP Reactivity: Human, Mouse WB, IF, IHC (p), FACS Host: Rabbit Polyclonal RB32966 unconjugated

anti-Synaptophysin (SYP) (C-Term) antibody
SYP Reactivity: Human WB, IF, ICC, IHC (p), IHC (fro), IHC (wm) Host: Rabbit Polyclonal unconjugated

anti-Synaptophysin (SYP) (AA 225-253), (C-Term) antibody
SYP Reactivity: Human, Mouse WB, IHC (p) Host: Rabbit Polyclonal RB32966 unconjugated

anti-Synaptophysin (SYP) (Internal Region) antibody
SYP Reactivity: Human, Rat, Mouse WB, IHC, IF, ICC, ELISA Host: Rabbit Polyclonal unconjugated

anti-Synaptophysin (SYP) antibody
SYP Reactivity: Human, Dog WB, IF Host: Mouse Monoclonal 5H2 unconjugated

anti-Synaptophysin (SYP) (N-Term) antibody
SYP Reactivity: Human, Rat, Mouse, Cow, Dog, Guinea Pig, Horse, Zebrafish (Danio rerio), Goat WB, IHC Host: Rabbit Polyclonal unconjugated

anti-Synaptophysin (SYP) (AA 1-313) antibody
SYP Reactivity: Human WB, ELISA Host: Mouse Monoclonal 3B3 unconjugated

anti-Synaptophysin (SYP) antibody
SYP Reactivity: Human, Rat WB, IHC, ELISA Host: Mouse Monoclonal 7H12 unconjugated

anti-Synaptophysin (SYP) antibody
Microarray verified SYP Reactivity: Human, Mouse IHC Host: Mouse Monoclonal MAB551901 unconjugated

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Colon: A moderate to strong staining of the axons of the Auerbach's and Meissner's plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.
Product Description: Synaptophysin antibody validated for immunohistochemistry on 76 different Normal Tissues
Negative Control: Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.

Protocol

Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 ° C. Antibody is stable for 24 months. Non- hazardous.

Expiry Date

24 months
Target

Synaptophysin (SYP)

Alternative Name

Synaptophysin

Background

Gene Alias: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH

UniProt

P08247

Pathways

Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity