Recombinant Synaptophysin antibody (AA 274-313)

Catalog No. ABIN7477577

The Rabbit Monoclonal anti-Synaptophysin antibody has been validated for IHC. It is suitable to detect Synaptophysin in samples from Human.

Quick Overview for Recombinant Synaptophysin antibody (AA 274-313) (ABIN7477577)

Target: Synaptophysin (SYP)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Synaptophysin antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-462R

Product Details anti-Synaptophysin Antibody

Binding Specificity: AA 274-313

Immunogen: Recombinant fragment (around aa 274-313) of human Synaptophysin (SYP) protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A moderate to strong staining of the axons of the Auerbach's and Meissner's plexus should be seen while the endocrine cells of the mucosa and the peripheral nerves in the mucosa must show an at least a weak to moderate staining. Pancreas: A strong staining should be seen in islets of Langerhans and in peripheral nerve fibres located between the exocrine cells.

Negative Control: Colon: All non-endocrine epithelial cells and lymphocytes should not show any staining.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Synaptophysin

Target: Synaptophysin (SYP)

Alternative Name: Synaptophysin

Background: Major synaptic vesicle protein p38, MRX96, MRXSYP, Syn p38, Syp, SYPH,Synaptophysin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P08247

Pathways: Regulation of G-Protein Coupled Receptor Protein Signaling, Regulation of long-term Neuronal Synaptic Plasticity