Recombinant TdT antibody

Catalog No. ABIN7477595

The Rabbit Monoclonal anti-TdT antibody has been validated for IHC. It is suitable to detect TdT in samples from Human.

Quick Overview for Recombinant TdT antibody (ABIN7477595)

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This TdT antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-453R

Product Details anti-TdT Antibody

Isotype: IgG

Application Details

Application Notes: IHC 1:75-1:150

Comment:

Positive Control: Thymus: At least a moderate nuclear immunostaining should be seen in virtually all cortical lymphocytes of the normal thymus.

Negative Control: Thymus: The vast majority of the medullary lymphocytes of the normal thymus should be negative. , Tonsil: The vast majority of lymphoid cells and all epithelial cells should be negative.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:100 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for TdT

Target: TdT (DNTT) (Deoxynucleotidyltransferase, terminal (DNTT))

Alternative Name: TdT

Background: Deoxynucleotidyltransferase terminal, DNTT, Nucleosidetriphosphate DNA Deoxynucleotidylexotransferase, TDT, Terminal addition enzyme, Terminal deoxynucleotidyltransferase, Terminal deoxyribonucleotidyltransferase,TdT antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P04053

Pathways: DNA Damage Repair