Recombinant Topoisomerase II alpha antibody (AA 1352-1493)

Catalog No. ABIN7477603

This anti-Topoisomerase II alpha antibody is a Rabbit Monoclonal antibody detecting Topoisomerase II alpha in IHC. Suitable for Human.

Quick Overview for Recombinant Topoisomerase II alpha antibody (AA 1352-1493) (ABIN7477603)

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Topoisomerase II alpha antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: 802R

Product Details anti-Topoisomerase II alpha Antibody

Binding Specificity: AA 1352-1493

Purpose: Topoisomerase 2-alpha / TOPO2A antibody (802R)

Immunogen: Recombinant human Topoisomerase II alpha fragment (aa1352-1493) (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A fraction of cells, especially in the crypt basis should show a moderate to strong nuclear TOPO2A immunostaining.

Product Description/Background: Topoisomerase 2-alpha / TOPO2A antibody validated for immunohistochemistry on 76 different Normal Tissues

Negative Control: Colon: Epithelial cells must not show any TOPO2A staining in the cytoplasm and in most nuclei of the surface epithelium.

Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 C. Antibody without azide - store at -20 to -80 C. Antibody is stable for 24 months. Non- hazardous. No MSD required.

Expiry Date: 24 months

Target Details for Topoisomerase II alpha

Target: Topoisomerase II alpha (TOP2A) (Topoisomerase (DNA) II alpha 170kDa (TOP2A))

Alternative Name: Topoisomerase 2-alpha

Background: Gene Alias: ATP hydrolyzing DNA topoisomerase II alfa, DNA gyrase, DNA topoisomerase (ATP hydrolyzing), DNA topoisomerase 2 alpha, DNA topoisomerase II 170kD, DNA topoisomerase II alpha, Topoisomerase DNA II alpha 170 kDa, TP2A

UniProt: P11388

Pathways: Cell Division Cycle, Mitotic G1-G1/S Phases