Recombinant Villin 1 antibody (AA 600-700)

Catalog No. ABIN7477617

This Rabbit Monoclonal antibody specifically detects Villin 1 in IHC. It exhibits reactivity toward Human.

Quick Overview for Recombinant Villin 1 antibody (AA 600-700) (ABIN7477617)

Target: Villin 1 (VIL1)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Monoclonal

Conjugate: This Villin 1 antibody is un-conjugated

Application: Immunohistochemistry (IHC)

Clone: MSVA-459R

Product Details anti-Villin 1 Antibody

Binding Specificity: AA 600-700

Immunogen: Recombinant fragment (around aa600-700) of human Villin protein (exact sequence is proprietary)

Isotype: IgG

Application Details

Application Notes: IHC 1:100-1:200

Comment:

Positive Control: Colon: A moderate to strong villin staining with predominance of the apical membrane should be seen in all epithelial cells. Liver: A weak to moderate staining of the apical pole of hepatocytes should be seen.

Negative Control: Colon: Villin staining should be absent in all non-epithelial cells.

Protocol: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C,-80 °C

Storage Comment: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.

Target Details for Villin 1

Target: Villin 1 (VIL1)

Alternative Name: Villin

Background: VIL1, Villin-1, Villin1,Villin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt: P09327

Pathways: EGFR Signaling Pathway, Regulation of Actin Filament Polymerization