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Recombinant Vimentin antibody (AA 2-466)

Name: Recombinant Vimentin antibody (AA 2-466)
Brand: antibodies-online
SKU: ABIN7477619
Price: 1454.93 USD
Availability: InStock

The Rabbit Monoclonal anti-Vimentin antibody has been validated for IHC. It is suitable to detect Vimentin in samples from Human.

endometrium proliferation (Recombinant Vimentin antibody (AA 2-466))

IHC

Duodenum mucosa (Recombinant Vimentin antibody (AA 2-466))

IHC

Larynx Strong vimentin positivity of the tumor stroma in a squamous cell carcinoma (Recombinant Vimentin antibody (AA 2-466))

IHC

3 images

Catalog No. ABIN7477619

Quick Overview for Recombinant Vimentin antibody (AA 2-466) (ABIN7477619)

Target

See all Vimentin (VIM) Antibodies

Vimentin (VIM)

Antibody Type

Recombinant Antibody

Reactivity

Human

Host

Rabbit

Clonality

Monoclonal

Conjugate

This Vimentin antibody is un-conjugated

Application

Immunohistochemistry (IHC)

Clone

MSVA-458R

Discover our top product VIM Primary Antibody

Binding Specificity
33

32

17

16

12

10

9

7

5

3

3

2

2

2

2

2

2

2

2

2

2

2

2

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1

1
AA 2-466

Immunogen

A recombinant fragment (around aa 2-466) of human Vimentin protein (exact sequence is proprietary)

Isotype

IgG
anti-Vimentin (VIM) (AA 371-466) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Cow, Monkey WB, ELISA, FACS, IHC (p), ICC, IHC (fro), IF (p), IF (cc) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, ELISA, IF, IHC (p), ICC, IP, IHC (fro) Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) (AA 435-466), (C-Term) antibody
VIM Reactivity: Human, Mouse, Rat WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse, Rat, Pig, Chicken WB, IHC (p), ICC, IP Host: Mouse Monoclonal VI-10 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Mammalian WB, FACS, IHC (p), ICC Host: Mouse Monoclonal VI-01 unconjugated

anti-Vimentin (VIM) (AA 1-200) antibody
VIM Reactivity: Human WB, IHC, IF Host: Rabbit Polyclonal unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human, Mouse WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB41034 unconjugated

anti-Vimentin (VIM) antibody
VIM Reactivity: Human WB, IHC, IF Host: Mouse Monoclonal C12 unconjugated

anti-Vimentin (VIM) (AA 430-457), (C-Term) antibody
VIM Reactivity: Human WB, IF, FACS, IHC (p) Host: Rabbit Polyclonal RB16691 unconjugated

anti-Vimentin (VIM) antibody
KO Validated VIM Reactivity: Human WB, IHC, IF, IP Host: Rabbit Monoclonal unconjugated

Application Notes

IHC 1:100-1:200

Comment

Positive Control: Liver: Kupffer cells must show a strong staining and sinusoidal endothelial cells should show an at least a weak staining. Colon: Endothelial and muscle cells of large vessels and stromal cells must show a strong Vimentin staining while dispersed intraepithelial T-cells must show an at least moderate intensity staining.
Negative Control: Liver: Hepatocytes cells must be Vimentin negative. Colon: All epithelial cells must be negative.

Protocol

Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121 °C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37 °C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer's directions.

Restrictions

For Research Use only
Format

Liquid

Storage

4 °C,-20 °C,-80 °C

Storage Comment

Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non- hazardous.
Target

Vimentin (VIM)

Alternative Name

Vimentin

Background

VIM,Vimentin antibody validated for immunohistochemistry on 76 different Normal Tissues

UniProt

P08670

Pathways

Caspase Cascade in Apoptosis