Borrelia Burgdorferi antibody (Peroxidase (POD))

Catalog No. ABIN7478142

The Rabbit Polyclonal anti-Borrelia Burgdorferi antibody is suitable to detect Borrelia Burgdorferi in samples from Borrelia burgdorferi. It has been validated for WB, IHC and EIA.

Quick Overview for Borrelia Burgdorferi antibody (Peroxidase (POD)) (ABIN7478142)

Target: Borrelia Burgdorferi

Reactivity: Borrelia burgdorferi

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Borrelia Burgdorferi antibody is conjugated to Peroxidase (POD)

Application: Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)

Product Details anti-Borrelia Burgdorferi Antibody (Peroxidase (POD))

Specificity: Reactive Western Blot bands at 83kD, 41kD, 34kD and 31kD + additional low MW bands

Cross-Reactivity (Details): Cross-reacts with Treponema pallidum, B. hermsii & B. parkerii

Purification: This product consists of purified IgG fraction of the neat antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3). Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. . Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

Immunogen: Whole cell preparation from B. burgdorferi

Isotype: IgG

Application Details

Application Notes: TITER : >1:1,000 in Western Blot. >1:40,000 in avidin-biotin IFA
This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays, Functions well in formalin fixed/paraffin embedded settings

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

Preservative: Thimerosal (Merthiolate)

Precaution of Use: This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.

Target Details for Borrelia Burgdorferi

Target: Borrelia Burgdorferi