Elementary Bodies antibody (Peroxidase (POD))
Quick Overview for Elementary Bodies antibody (Peroxidase (POD)) (ABIN7478217)
Target
Reactivity
Host
Clonality
Conjugate
Application
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Specificity
- Purified elementary bodies, disrupted
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Cross-Reactivity (Details)
- Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac
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Purification
- This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
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Immunogen
- L2 + other serovar groups
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Isotype
- IgG
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Application Notes
- TITER : >1:1,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
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Restrictions
- For Research Use only
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Format
- Liquid
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Concentration
- Lot specific
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Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
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Preservative
- Thimerosal (Merthiolate)
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Precaution of Use
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
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Storage
- 4 °C,-20 °C
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Storage Comment
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))
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Alternative Name
- Chlamydia trachomatis Elementary Bodies
Target
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