Elementary Bodies antibody (FITC)

Catalog No. ABIN7478220

The Rabbit Polyclonal anti-Elementary Bodies antibody is suitable to detect Elementary Bodies in samples from Chlamydia trachomatis. It has been validated for Func.

Quick Overview for Elementary Bodies antibody (FITC) (ABIN7478220)

Target: Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

Reactivity: Chlamydia trachomatis

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Elementary Bodies antibody is conjugated to FITC

Application: Functional Studies (Func)

Product Details anti-Elementary Bodies Antibody (FITC)

Specificity: Purified elementary bodies, disrupted

Cross-Reactivity (Details): Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac

Purification: This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

Immunogen: L2 + other serovar groups

Isotype: IgG

Application Details

Application Notes: TITER : 1:1,000-3,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: Lot specific

Buffer: The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.

Target Details for Elementary Bodies

Target: Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

Alternative Name: Chlamydia trachomatis Elementary Bodies