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Elementary Bodies antibody (Peroxidase (POD))

This anti-Elementary Bodies antibody is a Rabbit Polyclonal antibody detecting Elementary Bodies in IHC, WB and EIA. Suitable for Chlamydia trachomatis.
Catalog No. ABIN7478221

Quick Overview for Elementary Bodies antibody (Peroxidase (POD)) (ABIN7478221)

Target

Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

Reactivity

Chlamydia trachomatis

Host

  • 10
  • 8
Rabbit

Clonality

  • 16
Polyclonal

Conjugate

  • 6
  • 5
  • 4
  • 2
  • 1
This Elementary Bodies antibody is conjugated to Peroxidase (POD)

Application

  • 10
  • 6
  • 5
  • 5
  • 4
  • 3
  • 2
  • 1
  • 1
Immunohistochemistry (IHC), Western Blotting (WB), Enzyme Immunoassay (EIA)
  • Specificity

    Purified elementary bodies, disrupted

    Cross-Reactivity (Details)

    Cross-reacts with Chlamydia psittacii & Chlamydia pneumoniae (TWAR) UNINFECTED CELL REACTIVITY: Negative vs. HEp-2 cells and egg yolk sac

    Purification

    This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.

    Immunogen

    L2 + other serovar groups

    Isotype

    IgG
  • Application Notes

    TITER : 1:1,000-3,000 by indirect immunofluorescence vs. all serovars (A-K, L1-L3), This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !

    Preservative

    Thimerosal (Merthiolate)

    Precaution of Use

    This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
  • Target

    Elementary Bodies (EB) (Chlamydia trachomatis Elementary Bodies (EB))

    Alternative Name

    Chlamydia trachomatis Elementary Bodies
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