Clostridium Species antibody (Peroxidase (POD))
Reactivity: Clostridium
WB, IHC, EIA
Host: Rabbit
Polyclonal
Peroxidase (POD)
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- Target
- Clostridium Species
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Reactivity
- Clostridium
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Host
- Rabbit
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Clonality
- Polyclonal
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Conjugate
- Peroxidase (POD)
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Application
- Western Blotting (WB), Immunohistochemistry (IHC), Enzyme Immunoassay (EIA)
- Specificity
- Reactive with proteins from common Clostridia species
- Cross-Reactivity (Details)
- Antiserum is not absorbed & may react with related microorganisms, Reactive (IFA) with Cl. perfingens, Cl. sordellii, Cl. novyi, Cl. septicum, Cl. chauvoei
- Purification
- This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
- Immunogen
- Extract of Cl. perfingens, Cl. sordellii, Cl. novyi, Cl. septicum, Cl. chauvoei
- Isotype
- IgG
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- Application Notes
- TITER : >1:500 by indirect IFA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays.
- Restrictions
- For Research Use only
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- Format
- Liquid
- Concentration
- Lot specific
- Buffer
- This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
- Preservative
- Thimerosal (Merthiolate)
- Precaution of Use
- This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
- Storage
- 4 °C,-20 °C
- Storage Comment
- Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.
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- Target
- Clostridium Species
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