The Rabbit Polyclonal anti-CTXB antibody has been validated for EIA, WB and IHC. It is suitable to detect CTXB in samples from Vibrio cholerae.
Catalog No. ABIN7478349
Quick Overview for CTXB antibody (Peroxidase (POD)) (ABIN7478349)
Target
CTXB
(CtxB Gene Product (CTXB))
Reactivity
Vibrio cholerae
Host
Rabbit
Clonality
Polyclonal
Conjugate
This CTXB antibody is conjugated to Peroxidase (POD)
Application
Enzyme Immunoassay (EIA), Western Blotting (WB), Immunohistochemistry (IHC)
Specificity
Subunit of Cholera toxin
Cross-Reactivity (Details)
cross reacts with E. coli heat labile toxin
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled to a highly purified preparation of horseradish peroxidase (RZ >3) by methods. Care is taken to ensure adequate conjugation while preserving maximum enzyme activity. Free enzyme is absent. Immunoglobulin concentration in this product is 1-2 mg/ml. Estimated molar HRP:IgG substitution is 2-3.
TITER : > 1:1,000 by ELISA, This product is suitable for immunohistochemical, immunoblotting and EIA applications. Working strength must be determined by the user but a starting range of 1:20 - 1:200 is recommended for immunochemistry and blotting while 1:200 - 1:1,000 is recommended for enzyme immunoassays. immunoprecipitates toxin in gel techniques
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
This preparation is supplied in a phosphate saline buffer stabilized with bovine serum albumin (10 mg/mL) and preserved with 0.002 % thimerosal. NOTE: sodium azide is a potent inhibitor of peroxidase !
Preservative
Thimerosal (Merthiolate)
Precaution of Use
This product contains Thimerosal (Merthiolate) and Sodium azide: POISONOUS AND HAZARDOUS SUBSTANCES which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommended short term (<6 months) storage is liquid at 2-8°C. For longer term storage, aliquot and freeze.