The Rabbit Polyclonal anti-Vaccinia Virus antibody has been validated for Func. It is suitable to detect Vaccinia Virus in samples from Vaccinia Virus (VACV).
Catalog No. ABIN7478352
Quick Overview for Vaccinia Virus antibody (FITC) (ABIN7478352)
Target
Vaccinia Virus (VACV)
(Vaccina Virus (VACV))
Reactivity
Vaccinia Virus (VACV)
Host
Rabbit
Clonality
Polyclonal
Conjugate
This Vaccinia Virus antibody is conjugated to FITC
Application
Functional Studies (Func)
Specificity
Purified virions
Cross-Reactivity (Details)
Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox
Purification
This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.
TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).
Restrictions
For Research Use only
Format
Liquid
Concentration
Lot specific
Buffer
The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C,-20 °C
Storage Comment
Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.