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Vaccinia Virus antibody (FITC)

The Rabbit Polyclonal anti-Vaccinia Virus antibody has been validated for Func. It is suitable to detect Vaccinia Virus in samples from Vaccinia Virus (VACV).
Catalog No. ABIN7478352

Quick Overview for Vaccinia Virus antibody (FITC) (ABIN7478352)

Target

Vaccinia Virus (VACV) (Vaccina Virus (VACV))

Reactivity

  • 19
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Vaccinia Virus (VACV)

Host

  • 20
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Rabbit

Clonality

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Polyclonal

Conjugate

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  • 4
  • 1
This Vaccinia Virus antibody is conjugated to FITC

Application

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Functional Studies (Func)
  • Specificity

    Purified virions

    Cross-Reactivity (Details)

    Does not crossreact with Parainfluenza (1-3), RSV, Adeno, Influenza A&B or HSV1, This antibody is known to react with the B5R and the A33R proteins of the virus. UNINFECTED CELL REACTIVITY: Does not react with uninfected cells. Reactive with the Lister & MVA strains of Vaccinia and Monkeypox

    Purification

    This product consists of purified IgG fraction of the above antiserum covalently coupled with high purity Isomer I of fluorescein isothiocyanate. Care is taken to ensure complete removal of any free fluorescein from the final product.

    Immunogen

    New York City Board of Health (NYCBOH) strain

    Isotype

    IgG
  • Application Notes

    TITER : >1:1,000 by indirect ELISA. Functions in IFA, IHC & PRNT, Applications for this product include direct FA staining of target antigens in a permissive tissue culture system. Working dilutlion must be determined by the user for his or her application but a starting range of 1:10 - 1:50 is suggested. Acetone fixation of the antigen source is recommended prior to staining. We also recommend use of COUNTERSTAIN/BLOCKING DILUENT (Cat.#) and MOUNTING MEDIUM (Cat.#) for optimum product performance. Enzyme amplification following reaction with FITC conjugate can also be accomplished utilizing enzyme-antibody conjugates specific to FITC (Cat.#s & ).

    Restrictions

    For Research Use only
  • Format

    Liquid

    Concentration

    Lot specific

    Buffer

    The product is formulated in a phosphate saline buffer (0.01M, pH 7.2) containing bovine serum albumin (10 mg/mL) as a stabilizer and 0.1 % sodium azide preservative.

    Preservative

    Sodium azide

    Precaution of Use

    This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

    Storage

    4 °C,-20 °C

    Storage Comment

    Recommened short term (<6 months) storage is liquid at 2-8°C under subdued light. For longer term storage, aliquot and freeze.
  • Target

    Vaccinia Virus (VACV) (Vaccina Virus (VACV))

    Alternative Name

    Vaccina Virus

    Target Type

    Virus
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