Fibronectin antibody

Catalog No. ABIN7478856

This anti-Fibronectin antibody is a Rabbit Polyclonal antibody detecting Fibronectin in IF, ELISA, IHC (p), IHC (fro) and RIA. Suitable for Mouse.

Quick Overview for Fibronectin antibody (ABIN7478856)

Target: Fibronectin

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Fibronectin antibody is un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Radioimmunoassay (RIA)

Product Details anti-Fibronectin Antibody

Specificity: Mouse fibronectin

Cross-Reactivity (Details): Mouse

Characteristics: This antibody may be used to identify mouse fibronectin in experimental studies on wound healing, tumour progression, and tumour invasion, etc. Fibronectins are glycoproteins, which occur as dimers of two 200 kDa subunits. They have binding domains for bacterial proteins, collagens, heparin-like molecules and fibrin. Cellular fibronectin is widely distributed in the stroma of malignant tumours.

Purification: Protein G affinity purification

Immunogen: Purified fibronectin from murine plasma

Application Details

Application Notes: After de-waxing the tissue slices they are treated with 0.2 % hyaluronidase (app. 300 U/mg) in TBS 15 min at 37 °C. There after non-specific binding is blocked by blocking serum or 3 % BSA in TBS. For peroxidase systems blocking with 1 % peroxide solution in TBS for 30 min at RT is recommended.,Mouse skin,IFA, Immunohistochemistry on frozen and paraffin sections, ELISA, RIA,IHC 60 min at RT or 2-8 °C over night,IHC (frozen): 1:1000, ELISA:1:20000 (OD 0.5) Optimal working dilutions should, however, be determined by antibody titration.

Restrictions: For Research Use only

Handling

Concentration: 5 mg/mL

Buffer: Affinity purified (ProtG) antiserum lyophilized from phosphate buffered solution (non-sterile). Contains no BSA or sodium azide! Reconstitute with 0.5 mL of sterile water and store aliquots at -20 °C. For further dilution use appropriate antibody diluent, e.g. Art. No. PU002 for IHC.

Preservative: Without preservative

Storage: -20 °C

Storage Comment: -20°C

Target Details for Fibronectin

Target: Fibronectin