Recombinant Fc gamma RII (CD32) antibody

Catalog No. ABIN7487694

The Rabbit Chimeric anti-Fc gamma RII antibody is suitable to detect Fc gamma RII in samples from Human. It has been validated for IF, ELISA, IP, BR and InhA.

Quick Overview for Recombinant Fc gamma RII (CD32) antibody (ABIN7487694)

Target: Fc gamma RII (CD32)

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: Un-conjugated

Application: Immunofluorescence (IF), ELISA, Immunoprecipitation (IP), Blocking Reagent (BR), Inhibition Assay (InhA)

Clone: IV-3

Product Details

Purpose: Anti-CD32 [IV.3], Rabbit IgG, kappa

Specificity:

This antibody recognizes and binds CD32a.

Application Notes original Clone: This antibody successfully precipitated a 40-kd FcR from lysates of U937 and K562 cells but not from Daudi or Raji cells. It selectively inhibited the binding of murine IgG1-coated erythrocytes to U937 cells. IV.3 blocked mIgG1 anti-T3 (Leu-4) stimulation but not mIgG2a anti-T3 (OKT3) stimulation of T cells (PMID: 2936805). Anti-FcyRII antibody IV.3 specifically stained Ltk- cells transfected with the FcyR cDNA clone in an immunofluorescence assay (PMID: 2824655). It was reported that IV.3 induces anaphylaxis in immunocompetent CD32a-transgenic ""FCGR2A"" mice and that IV.3 also induce severe thrombocytopenia in FCGR2A mice (PMID: 26396093). IV.3, reacted with CD32A-R131 exclusively, confirming the specificity in an ELISA (PMID:17386079).

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG2b format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: The original antibody was generated against the FcR of K562 cells.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG2b

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details

Target: Fc gamma RII (CD32)

Alternative Name: CD32

Background: CDw32, Fc-gamma RII-a, FcγRIIa, FcγRII, FcRII-a, FCGR2A, IgG Fc receptor II-a, Low affinity immunoglobulin gamma Fc region receptor II-a

UniProt: P12318