Recombinant CD34 antibody

Catalog No. ABIN7487696

This Rabbit Chimeric antibody specifically detects CD34 in FACS, WB and IF. It exhibits reactivity toward Human.

Quick Overview for Recombinant CD34 antibody (ABIN7487696)

Target: CD34

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CD34 antibody is un-conjugated

Application: Flow Cytometry (FACS), Western Blotting (WB), Immunofluorescence (IF)

Clone: 2E 10

Product Details anti-CD34 Antibody

Purpose: Anti-CD34 [2E 10], Rabbit IgG, kappa

Specificity: The antibody is specific to a class III epitope on CD34, which is not susceptible to cleavage by neuraminidase, chymopapain or glycoprotease.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG3

Purification: Protein A affinity purified

Immunogen: The antibody was raised by repeatedly immunising BALB/c mice with KG-1a cells.

Isotype: IgG kappa

Application Details

Application Notes: This antibody, labelled with fluoroscein isothiocyanate, has been used in flow cytometry to assess the loss of expression of CD34 epitopes following enzyme incubation (Qian et al., 2008). The antibody has been used as a primary antibody against CD34 in Western blot analysis, using HRP-conjugated goat anti-mouse IgG as a secondary antibody.

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG3 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CD34

Target: CD34

Alternative Name: CD34

Background: Hematopoietic progenitor antigen, Hematopoietic progenitor cell antigen CD34

UniProt: P28906