Recombinant CRP antibody

Catalog No. ABIN7487718

The Rabbit Chimeric anti-CRP antibody has been validated for WB and ELISA. It is suitable to detect CRP in samples from Human.

Quick Overview for Recombinant CRP antibody (ABIN7487718)

Target: CRP (C-Reactive Protein (CRP))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This CRP antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Clone: P03

Product Details anti-CRP Antibody

Purpose: Anti-CRP [P03], Rabbit IgG, kappa

Specificity: This antibody reacts with human C reactive protein, which is a pentameric protein found in blood plasma. It is an acute phase reactive protein of hepatic origin. It is an important defense molecule for the body and its plasma concentration increases sharply during tissue damage, inflammation and bacterial infection. It promotes agglutination, bacterial capsular swelling, phagocytosis and complement fixation through its calcium-dependent binding to phosphorylcholine. It can interact with DNA and histones and may scavenge nuclear material released from damaged circulating cells.

Characteristics:

Original Species of Ab: Mouse

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was generated by immunizing BALB/c mice with C-reactive protein extracted from human plasma.

Isotype: IgG kappa

Application Details

Application Notes: Indirect ELISA was used for the primary screening of the hybridomas showing selective binding for human CRP. Spicy Root peroxidase labeled scFv P03 was used for the identification of natural CRP extracted from denatured and non-denatured blood in a western blot assay. This antibody in combination with P24 was also used in the double antibody sandwich detection of natural human CRP. The combination of P03 and P24 antibody was also used for the generation of time-resolved fluorescence immunoassay card for detection of C-reactive protein (CN105842440).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for CRP

Target: CRP (C-Reactive Protein (CRP))

Alternative Name: CRP

Background: C reactive protein, PTX1, C-reactive protein

UniProt: P02741

Pathways: Carbohydrate Homeostasis