Recombinant MAGEA3 antibody

Catalog No. ABIN7487738

This Rabbit Chimeric antibody specifically detects MAGEA3 in WB, ELISA, IHC, FACS and IP. It exhibits reactivity toward Human.

Quick Overview for Recombinant MAGEA3 antibody (ABIN7487738)

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Antibody Type: Recombinant Antibody

Reactivity: Human

Host: Rabbit

Clonality: Chimeric

Conjugate: This MAGEA3 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS), Immunoprecipitation (IP)

Clone: 21B4

Product Details anti-MAGEA3 Antibody

Purpose: Anti-MAGEA3 [21B4], Rabbit IgG, kappa

Specificity: This antibody binds human MAGEA3 and MAGEA6, but does not cross react with other members of the MAGE family like MAGE-A1, MAGE-A4 and MAGE-A10. MAGE-A3 is a tumor specific antigen and it is expressed on many tumors like melanoma, non-small cell lung cancer, hematologic malignancies, and others.

Characteristics:

Original Species of Ab: Human

Original Format of Ab: IgG1

Purification: Protein A affinity purified

Immunogen: The original antibody was isolated from a patient suffering from a tumor expressing a tumor specific antigen like MAGE-A3.

Isotype: IgG kappa

Application Details

Application Notes: The binding specificity of this antibody towards MAGE-A3 was determined using ELISA. The original antibody cross reacts with MAGEA6 but does not react with other members of the MAGE family in an ELISA or western blot assay. This antibody was used in the detection of HEK293T cells expressing MAGEA3 and MAGEA6 using flow cytometry. The original IgG1 antibody was capable of immunoprecipitating MAGE-A3 from lysates of SK-MEL-37 cells endogenously expressing MAGEA3. The original antibody was also used in the immunohistochemical staining of tissues form normal testis and squamous cell carcinoma of the lung (US20140093514).

Comment:

This chimeric rabbit antibody was made using the variable domain sequences of the original Human IgG1 format for improved compatibility with existing reagents assays and techniques.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for MAGEA3

Target: MAGEA3 (Melanoma Antigen Family A, 3 (MAGEA3))

Alternative Name: MAGEA3

Background: MAGE-3, MAGE3, MAGE 3, CT1.3, MAGE-3 antigen, Melanoma-associated antigen 3, Antigen MZ2-D, Cancer/testis antigen 1.3, MAGEA6, MAGE6, Melanoma-associated antigen 6, Cancer/testis antigen 1.6, CT1.6, MAGE-6 antigen, MAGE3B antigen

UniProt: P43357, P43360