Recombinant PROC antibody (Cleavage Site)

Catalog No. ABIN7487754

The Rabbit Chimeric anti-PROC antibody is suitable to detect PROC in samples from Human and Baboon. It has been validated for ELISA, BP and Purif.

Quick Overview for Recombinant PROC antibody (Cleavage Site) (ABIN7487754)

Target: PROC (Vitamin K-dependent protein C (PROC))

Antibody Type: Recombinant Antibody

Reactivity: Human, Baboon

Host: Rabbit

Clonality: Chimeric

Conjugate: This PROC antibody is un-conjugated

Application: ELISA, Blocking Peptide (BP), Purification (Purif)

Clone: HPC-4

Product Details anti-PROC Antibody

Binding Specificity: Cleavage Site

Purpose: Anti-Protein C [HPC-4], Rabbit IgG, kappa

Specificity:

Specifically binds to a specific twelve peptide sequence (EDQVDPRLIDGK) in the activation region of the Protein C (spanning the thrombin cleavage site). Does not react with activated protein C. Has been shown to be cross-reactive with baboon Protein C but not with bovine or mouse Protein C.

Application Notes original Clone: HPC-4 binds human protein C only in the presence of Ca²⁺, it binds to the reduced carboxymethylated protein C heavy chain, and it blocks protein C activation but does not bind or inactivate protein C or activated protein C bound to its plasma inhibitor. Furthermore, it is an anti-epitope tag antibody, as it recognizes the EDQVDPRLIDGK sequence (Taylor Jr et al., 1987, 3102560). It has been shown to work in ELISAs to determine expression levels (Velander et al., 1992, PMID: 1465430). HPC-4 has been shown to be effective at purifying proteins tagged with its epitope (Rezaie et al., 1992, PMID: 1283093). It has also been used to block the activation of Protein C by the thrombin-thrombomodulin complex (Stearns et al., 1988, PMID: 2447082) and was used for the same purpose in Baboons to lower the threshold of sepsis (Taylor Jr et al., 1987, PMID: 3102560). HPC-4 has been used in many other studies to observe the function of Protein C by knocking out its function in a Ca²⁺-dependent manner, purification, and others (Tanaka et al., 2006, PMID: 16398653) (Bae et al., 2007, PMID: 17823308).

Cross-Reactivity: Baboon

Characteristics: This chimeric rabbit antibody was made using the variable domain sequences of the original Mouse IgG1 format, for improved compatibility with existing reagents, assays and techniques.

Purification: Protein A affinity purified

Immunogen: This antibody was raised by immunising mice with human protein C.

Isotype: IgG, kappa

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Comment:

OriginalSpeciesName: Mouse

OriginalFormat: IgG1

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: PBS with 0.02 % Proclin 300.

Preservative: ProClin

Precaution of Use: This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.

Target Details for PROC

Target: PROC (Vitamin K-dependent protein C (PROC))

Alternative Name: Protein C

Background: Autoprothrombin IIA, C-tag, Protein C tag, Protein C-tag, HPC4, Vitamin K-dependent protein C, Anticoagulant protein C, Blood coagulation factor XIV, PROC

UniProt: P04070