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Recombinant T Cell Receptor antibody

Reactivity: Mouse FACS, IP, Crys, IHC Host: Rabbit Chimeric H57-597 unconjugated Recombinant Antibody
Catalog No. ABIN7487760
  • Target
    T Cell Receptor
    Antibody Type
    Recombinant Antibody
    Reactivity
    • 4
    • 1
    Mouse
    Host
    • 2
    • 2
    • 1
    Rabbit
    Clonality
    • 4
    • 1
    Chimeric
    Conjugate
    • 5
    Un-conjugated
    Application
    • 5
    • 4
    • 3
    • 2
    • 2
    • 1
    • 1
    Flow Cytometry (FACS), Immunoprecipitation (IP), Crystallization (Crys), Immunohistochemistry (IHC)
    Characteristics

    OriginalSpeciesName: Hamster, Armenian

    OriginalFormat: IgG

    Purification
    Purified antibody.
    Immunogen
    Hamsters were immunised with affinity purified DO-11.10 TCR, and produced detectable anti-TCR antibodies which were judged by the ability of the anti-sera to immunoprecipitate TCR from surface labelled DO-11.10 T cell hybridomas. Hamster 57 was selected for hybridisation, and B cell hybridomas were produced using polyethylene glycol fusion of hamster splenic B cells to the mouse myeloma variant. Selected mAb were screened after subsequent culturing for their ability to immunoprecipitate TCR from detergent lysates of surface-radiolabelled murine T cell hybridomas.
    Clone
    H57-597
    Isotype
    IgG lambda
  • Application Notes
    Optimal working dilution should be determined by the investigator.
    Comment

    This chimeric rabbit antibody was made using the variable domain sequences of the original Hamster IgG format, for improved compatibility with existing reagents, assays and techniques.

    Restrictions
    For Research Use only
  • Buffer
    PBS with 0.02 % Proclin 300.
    Preservative
    ProClin
    Precaution of Use
    This product contains ProClin: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
    Storage
    4 °C,-20 °C
    Storage Comment
    Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C.
  • Target
    T Cell Receptor
    Alternative Name
    TCR
    Synonyms
    Gm13948 antibody, Gm193 antibody, Gm4 antibody, OTTMUSG00000015160 antibody, TCR antibody, T cell receptor alpha variable 6-3 antibody, Trav6-3 antibody
    Background
    TCR-beta, T cell receptor, T-cell receptor, T cell receptor beta chain, TCRB, TRB, TCB1, Ti
    UniProt
    P01852
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