SIRPA antibody (FITC)

Catalog No. ABIN7505868

This Mouse Monoclonal antibody specifically detects SIRPA in FACS. It exhibits reactivity toward Human.

Quick Overview for SIRPA antibody (FITC) (ABIN7505868)

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This SIRPA antibody is conjugated to FITC

Application: Flow Cytometry (FACS)

Clone: 15-414

Product Details anti-SIRPA Antibody (FITC)

Purpose: Anti-Hu CD172a FITC

Specificity: The mouse monoclonal antibody 15-414 recognizes en extracellular epitope of CD172a (SIRP alpha), an approximately 90 kDa transmembrane glycoprotein expressed on cells of myeloid origin and neurons.

Purification: Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.

Immunogen: Kg-1a cell line

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μL reagent / 100 μL of whole blood or 10⁶ cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Restrictions: For Research Use only

Handling

Buffer: Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.

Target Details for SIRPA

Target: SIRPA (Signal-Regulatory Protein alpha (SIRPA))

Alternative Name: CD172a

Background: Signal regulatory protein alpha,CD172a, the signal-regulatory protein alpha (SIRP alpha), also known as SH2 domain-containing phosphatase substrate-1 (SHPS1), is a 75-110 kDa transmembrane glycoprotein expressed mainly on granulocytes, monocytes, macrophages, dendritic cells and neurons. Its extracellular ligand is CD47. CD172a serves as a substrate of activated receptor tyrosine kinases and upon phosphorylation it recruits SH2 domain-containing tyrosine phosphatases, thereby regulating signal transduction processes related to cell activation, transmigration and phagocytosis. CD172a is a specific marker of cardiomyocytes derived from human pluripotent stem cells and serves as a negative regulator of signaling and growth in myeloid progenitor cells.,PTPNS1, BIT, MFR, SIRPA, SHPS1

Gene ID: 140885

UniProt: P78324