CD235a/GYPA antibody (Biotin)

Catalog No. ABIN7505887

The Mouse Monoclonal anti-CD235a/GYPA antibody is suitable to detect CD235a/GYPA in samples from Human and Rat. It has been validated for FACS, IHC (p) and IHC (fro).

Quick Overview for CD235a/GYPA antibody (Biotin) (ABIN7505887)

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Reactivity: Human, Rat

Host: Mouse

Clonality: Monoclonal

Conjugate: This CD235a/GYPA antibody is conjugated to Biotin

Application: Flow Cytometry (FACS), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro))

Clone: JC159

Product Details anti-CD235a/GYPA Antibody (Biotin)

Purpose: Anti-Hu CD235a Biotin

Characteristics: The mouse monoclonal antibody JC159 recognizes an epitope between amino acids 27 and 40 of the extracellular portion of CD235a (glycophorin A), a sialoglycoprotein expressed on early erythroblasts, late erythroblasts, erythroblasts, mature erythrocytes and the cells of erythroid cell lines K562 and HEL. The antibody does not react with glycophorin B.

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: Membrane preparation from splenic hairy cell leukemia

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for CD235a/GYPA

Target: CD235a/GYPA (GYPA) (Glycophorin A (GYPA))

Alternative Name: CD235a

Background: Glycophorin A (MNS blood group),CD235a (glycophorin A, GPA) is a transmembrane sialoglycoprotein expressed on erythrocytes and their precursors. Similarly to CD235b (glycophorin B, GPB), these molecules provide the cells with a large mucin-like surface, which minimalizes aggregation between erythrocytes in the circulation. GPA is the carrier of blood group M and N specificities, while GPB accounts for S, s and U specificities. CD235a is a receptor of Hsa, a Streptococcus adhesin.,Glycophorin A, GYPA, GPA, PAS-2, Sialoglycoprotein alpha, MN sialoglycoprotein, GPSAT, GPErik, MSN blood group

Gene ID: 2993

UniProt: P02724

Pathways: Maintenance of Protein Location