N-Cadherin antibody (Extracellular Domain)

Catalog No. ABIN7505914

The Mouse Monoclonal anti-N-Cadherin antibody is suitable to detect N-Cadherin in samples from Human and Mouse. It has been validated for WB, IHC, IP, FACS and ICC.

Quick Overview for N-Cadherin antibody (Extracellular Domain) (ABIN7505914)

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Reactivity: Human, Mouse

Host: Mouse

Clonality: Monoclonal

Conjugate: This N-Cadherin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Flow Cytometry (FACS), Immunocytochemistry (ICC)

Clone: 8C11

Product Details anti-N-Cadherin Antibody

Binding Specificity: Extracellular Domain

Purpose: Anti-CD325 Purified

Characteristics: The mouse monoclonal antibody 8C11 recognizes an extracellular epitope of CD325 (N-cadherin), a transmembrane protein associated with actin cytoskeleton system, which is expressed mainly by neurons, osteoblasts, endothelial cells, and stem cells.

Purification: Purified by protein-A affinity chromatography.

Immunogen: bacterially expressed extracellular domain of human CD325

Isotype: IgG1, kappa

Application Details

Application Notes: Flow cytometry: Recommended dilution: 1-4 μg/mL.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Handling Advice: Do not freeze

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for N-Cadherin

Target: N-Cadherin (CDH2) (Cadherin 2 (CDH2))

Alternative Name: CD325

Background: Cadherin 2,CD325 (N-cadherin) is a type I transmembrane protein, which forms a complex with catenins, that is linked to the actin cytoskeleton. This complex is important in synapses and for functional plasticity of neurons, and is also essential for embryonic development. Decreased CD325 cleavage caused by mutations in presenilin 1 is associated with Alzheimer´s disease. Besides nervous system, CD325 is expressed on the surface of malignant T cells, and increases their adhesion to epithelia, as well as their ability to invade and metastasize to inflammatory sites.,N-Cadherin, cadherin 2, CDHM, CDH2

Gene ID: 1000

UniProt: P19022

Pathways: Regulation of Muscle Cell Differentiation, Cell-Cell Junction Organization, Synaptic Membrane