Cyclin D1 antibody (AA 1-295) (Biotin)

Catalog No. ABIN7505972

This anti-Cyclin D1 antibody is a Mouse Monoclonal antibody detecting Cyclin D1 in WB, IP, ICC, IHC (p), IHC (fro) and ICFC. Suitable for Human, Mouse, Rat and Non-Human Primate.

Quick Overview for Cyclin D1 antibody (AA 1-295) (Biotin) (ABIN7505972)

Target: Cyclin D1 (CCND1)

Reactivity: Human, Mouse, Rat, Non-Human Primate

Host: Mouse

Clonality: Monoclonal

Conjugate: This Cyclin D1 antibody is conjugated to Biotin

Application: Western Blotting (WB), Immunoprecipitation (IP), Immunocytochemistry (ICC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Immunohistochemistry (Frozen Sections) (IHC (fro)), Intracellular Flow Cytometry (ICFC)

Clone: DCS-6

Product Details anti-Cyclin D1 Antibody (Biotin)

Binding Specificity: AA 1-295

Purpose: Anti-Cyclin D1 Biotin

Specificity: The mouse monoclonal antibody DCS-6 recognizes cyclin D1, an ubiquitously expressed 33 kDa intracellular protein that migrates as a 36 kDa band under reducing SDS-PAGE conditions.

No Cross-Reactivity: Cat, Sheep

Purification: Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography.

Immunogen: recombinant human cyclin D1 (amino acids 1-295)

Isotype: IgG2a

Application Details

Application Notes: Flow cytometry: Recommended dilution: 2-10 μg/mL. Intracellular staining.
Immunohistochemistry: Recommended dilution: 5-10 μg/mL.

Restrictions: For Research Use only

Handling

Concentration: 1 mg/mL

Buffer: Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8°C. Do not freeze.

Target Details for Cyclin D1

Target: Cyclin D1 (CCND1)

Alternative Name: Cyclin D1

Background: Cyclin D1,Cyclin D1 (PRAD1, Bcl-1) is a cytoplasmic and nuclear protein, which is synthesized during G1 phase and assembles with either cyclin-dependent kinase 4 (CDK4) or CDK6 in response to growth factor stimulation. D-type cyclin-CDK complexes act to inactivate the growth-suppressive function of the Rb protein through its phosphorylation, and titrate CDK inhibitors such as p21Cip1 and p27Kip1. Whereas during G1 phase cyclin D1 accumulates in the nucleus, it translocates into the cytoplasm during S phase. Without growth factor-mediated stimulation, cyclin D1 is unstable, and undergoes ubiquitin-mediated degradation, which is triggered by its phosphorylation. Cyclin D1 destabilization participates in G1/S phase arrest.,CYC-D1, CCND1, Bcl-1, PRAD1, U21B31

Gene ID: 595

UniProt: P24385

Pathways: PI3K-Akt Signaling, Cell Division Cycle, Mitotic G1-G1/S Phases, ER-Nucleus Signaling