Vimentin antibody

Catalog No. ABIN7536784

This Sheep Polyclonal antibody specifically detects Vimentin in WB, ELISA, IP, IEP, Neut and IDe. It exhibits reactivity toward Human.

Quick Overview for Vimentin antibody (ABIN7536784)

Target: Vimentin (VIM)

Reactivity: Human

Host: Sheep

Clonality: Polyclonal

Conjugate: This Vimentin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunoprecipitation (IP), Immunoelectrophoresis (IEP), Neutralization (Neut), Immunodepletion (IDe)

Product Details anti-Vimentin Antibody

Purpose: Vimentin Polyclonal Antibody

Specificity: Human vimentin, VIM

Purification: Fractionation of IgG from antiserum

Immunogen: Human vimentin purified from plasma

Isotype: IgG

Application Details

Application Notes: Immunoblot 5-25 μg/mL, ELISA capture 20-50 μg/mL

Comment:

Whole IgG is a base level product and refers to IgG antibodies purified from antiserum using multiple precipitation and dialysis steps. The purity of the IgG is typically 90% and is provided in a solution of HEPES buffered saline containing 50% glycerol (v/v). The titre is essentially the same as the starting antiserum and each vial typically contains the amount of IgG recovered from one millilitre of antiserum. This level of product is generally intended for use in applications such as immunoprecipitation, immunoelectrophoresis, immuno-depletion and activity neutralization assays.

Restrictions: For Research Use only

Handling

Format: Liquid

Reconstitution: Allow product to warm to room temperature and gently mix before use

Concentration: 10 mg/mL

Buffer: 10 mM HEPES, pH 7.4, 150 mM NaC, 50 % (v/v) glycerol

Storage: -20 °C

Storage Comment: Store between -10°C and -20°C

Target Details for Vimentin

Target: Vimentin (VIM)

Alternative Name: Vimentin

Pathways: Caspase Cascade in Apoptosis