P80 (C-Term), (Catalytic Domain) antibody

Catalog No. ABIN7538839

This Mouse Monoclonal antibody specifically detects P80 in HIER, IHC and IHC (p). It exhibits reactivity toward Human.

Quick Overview for P80 (C-Term), (Catalytic Domain) antibody (ABIN7538839)

Target: P80

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Application: Heat-induced Epitope Retrieval (HIER), Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))

Clone: 5A4

Product Details

Binding Specificity: C-Term, Catalytic Domain

Purpose: Liquid Mouse Monoclonal Antibody p80 (Anaplastic Lymphoma Kinase)

Specificity: Human ALK protein and C-terminal ALK fusion proteins containing the immunized target sequence.

Characteristics: This product is intended for the qualitative identification by light microscopy of human p80 protein in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to a region which spans the tyrosine kinase catalytic domain and part of the C-terminus of the NPM-ALK transcript (419-520alphaalpha).

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:200 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 32 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details

Target: P80