AMACR antibody

Catalog No. ABIN7538840

The Mouse Monoclonal anti-AMACR antibody has been validated for IHC, IHC (p) and HIER. It is suitable to detect AMACR in samples from Human.

Quick Overview for AMACR antibody (ABIN7538840)

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This AMACR antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: EPMU1

Product Details anti-AMACR Antibody

Purpose: Liquid Mouse Monoclonal Antibody Alpha-Methylacyl-CoA Racemase (AMACR, p504S)

Specificity: Human alpha-methylacyl-CoA racemase.

Characteristics: This product is intended for the qualitative identification by light microscopy of alpha-methylacyl-CoA racemase (AMACR) molecules in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Prokaryotic recombinant protein corresponding to 382 amino acids of the human alpha-methylacyl-CoA racemase molecule.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Suggested dilution: 1:200 for 30 minutes at 25°C. Heat induced epitope retrieval using Epitope Retrieval Solution pH 9.0. This is provided as a guide and users should determine their own optimal working dilutions. Technical note: When utilised in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognized. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 % . If these recommendations are not followed, staining intensity will be reduced. It is not recommended for use with PBS, since the use of PBS based wash buffers and possibly PBS based antibody diluents gives increased background staining and decreased staining intensity. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 84 mg/L

Buffer: This product is a liquid tissue culture supernatant containing 15 mM sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for AMACR

Target: AMACR (alpha-Methylacyl-CoA Racemase (AMACR))

Alternative Name: Alpha-Methylacyl-CoA Racemase

Pathways: Monocarboxylic Acid Catabolic Process