MUC16 antibody

Catalog No. ABIN7538844

The Mouse Monoclonal anti-MUC16 antibody (Clone Ov185:1) (ABIN7538844) specifically detects MUC16 in IHC, IHC (p) and HIER. The antibody is reactive with Human samples.

Quick Overview for MUC16 antibody (ABIN7538844)

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This MUC16 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: Ov185:1

Product Details anti-MUC16 Antibody

Purpose: Liquid Mouse Monoclonal Antibody CA125 (Ovarian Cancer Antigen)

Specificity: Repetitive protein determinant expressed in the protein core of the CA125 human ovarian cancer antigen.

Characteristics: This product is intended for the qualitative identification by light microscopy of CA125 (Ovarian Cancer Antigen) molecules in paraffin sections.

Purification: Purified IgG fraction

Immunogen: A partially purified mucin fraction from a pool of cancer tissue from patients with epithelial ovarian cancer.

Isotype: IgG1

Application Details

Application Notes: Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 12.5 mg/L

Buffer: This product is a purified IgG fraction presented in phosphate-buffered saline ( pH 7.6) with 1 % bovine serum albumin carrier protein and containing 15 mM sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for MUC16

Target: MUC16 (CA125) (Mucin 16, Cell Surface Associated (CA125))

Alternative Name: CA125