This product is intended for the qualitative identification by light microscopy of Immunoglobulin D in paraffin sections.
Purification
Tissue culture supernatant
Immunogen
Prokaryotic recombinant protein corresponding to 222 amino acids of the N terminus of the delta heavy chain constant region of the human immunoglobulin D molecule.
GMP Grade
Reactivity: Human
FACS
Host: Mouse
Monoclonal
IA6-2
iFluor™700
Application Notes
Immunohistochemistry on paraffin sections. Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 9. Suggested dilution: 1:1000 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Technical Note: The use of PBS-based diluents may result in increased background staining. Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.
Restrictions
For Research Use only
Format
Liquid
Concentration
133 mg/L
Buffer
This product is a liquid tissue culture supernatant containing sodium azide as a preservative.
Preservative
Sodium azide
Precaution of Use
This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.
Storage
4 °C
Storage Comment
Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.