PAX5 antibody (C-Term)

Catalog No. ABIN7538922

This anti-PAX5 antibody is a Mouse Monoclonal antibody detecting PAX5 in IHC, IHC (p) and HIER. Suitable for Human.

Quick Overview for PAX5 antibody (C-Term) (ABIN7538922)

Target: PAX5 (Paired Box 5 (PAX5))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This PAX5 antibody is un-conjugated

Application: Immunohistochemistry (IHC), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)), Heat-induced Epitope Retrieval (HIER)

Clone: 1EW

Product Details anti-PAX5 Antibody

Binding Specificity: C-Term

Purpose: Liquid Mouse Monoclonal Antibody Pax-5

Specificity: Human Pax-5.

Characteristics: This product is intended for the qualitative identification by light microscopy of human Pax-5 in paraffin sections.

Purification: Tissue culture supernatant

Immunogen: Recombinant protein corresponding to part of the C-terminus of the human Pax-5 molecule.

Isotype: IgG1

Application Details

Application Notes: Heat Induced Epitope Retrieval (HIER): Epitope Retrieval Solution pH 6. Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should determine their own optimal working dilutions. Technical note: When utilised in immunohistochemistry, the use of H2O2 to block endogenous peroxidase has been shown to have a detrimental effect on the epitope recognized. It is, therefore, CRITICAL that blocking with H2O2 should be carried out after application of the primary antibody with solutions of no greater than 3 % . If these recommendations are not followed, staining intensity will be reduced.,Immunohistochemical (IHC) staining techniques allow for the visualization of antigens via the sequential application of a specific antibody to the antigen (primary antibody), a secondary antibody to the primary antibody and an enzyme complex with a chromogenic substrate with interposed washing steps. The enzymatic activation of the chromogen results in a visible reaction product at the antigen site. The specimen may then be counterstained and coverslipped. Results are interpreted using a light microscope.

Restrictions: For Research Use only

Handling

Format: Liquid

Concentration: 29 mg/L

Buffer: This product is a liquid tissue culture supernatant containing sodium azide as a preservative.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C

Storage Comment: Store at 2-8 °C. Do not freeze. Return to 2-8 °C immediately after use. Do not use after expiration date indicated on the vial label. Storage conditions other than those specified above must be verified by the user.

Expiry Date: 12 months

Target Details for PAX5

Target: PAX5 (Paired Box 5 (PAX5))

Alternative Name: Pax-5