Emilin1 antibody

Catalog No. ABIN7539245

The Mouse Monoclonal anti-Emilin1 antibody (Clone 1H2-G8) (ABIN7539245) specifically detects Emilin1 in WB, IHC, ELISA and IF. The antibody is reactive with Human samples.

Quick Overview for Emilin1 antibody (ABIN7539245)

Target: Emilin1 (EMILIN1) (Elastin Microfibril Interfacer 1 (EMILIN1))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This Emilin1 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), ELISA, Immunofluorescence (IF)

Clone: 1H2-G8

Product Details anti-Emilin1 Antibody

Purpose: Emilin-1 antibody

Purification: Protein-G purified

Immunogen: recombinant human Emilin-1

Isotype: IgG2a

Application Details

Application Notes: Optimal working dilution should be determined by the investigator.

Restrictions: For Research Use only

Handling

Format: Lyophilized

Reconstitution: Centrifuge vial prior to opening. Reconstitute in sterile water to a concentration of 0.1-1.0 mg/mL.

Buffer: PBS

Storage: 4 °C,-20 °C

Storage Comment: The lyophilized antibody is stable for at least 2 years at -20°C. After sterile reconstitution the antibody is stable at 2-8°C for up to 6 months. Frozen aliquots are stable for at least 6 months when stored at -20°C. Addition of a carrier protein or 50% glycerol is recommended for frozen aliquots.

Expiry Date: 24 months

Target Details for Emilin1

Target: Emilin1 (EMILIN1) (Elastin Microfibril Interfacer 1 (EMILIN1))

Alternative Name: Emilin-1

Background: Elastin microfibril interface-located protein 1, Elastin microfibril interfacer 1, EMI,Emilins (elastin microfibril interface located proteins) are extracellular matrix glycoproteins that localize to sites with proximity to elastin and microfibrils. They consist of an N-terminal cysteine-rich EMI domain and a conserved C-terminal gC1q-like domain. Emilin-1 is abundant in elastin-rich tissues such as blood vessels, skin, heart and lung. It influences placenta formation and initial organogenesis with a later role in interstitial connective tissue. Emilin-2 is larger than Emilin-1 and contains a unique proline-rich domain. It is likely involved in the process of elastogenesis. Multimerin-2 (also known as Emilin-3 or EndoGlyx-1) is expressed during embryonic development. Multimerin-1 (also known as Emilin-4) is expressed in platelets and the endothelium of blood vessels and may act as a carrier protein for platelet factor V. Emilin-5 is encoded by the Emilin-3 gene and is sometimes referred to as Emilin-3. It contains the N-terminal cysteine-rich EMI domain but lacks the C-terminal gC1q-like domain. Emilin-1 have been shown to be expressed in smooth muscle and other mesenchymal tissues and is localized at the proximity of elastin and microfibrils. Emilin-1 exerts an important role in lymphatic system, being a crucial structural molecule that regulates the formation of lymphatic capillaries and collectors. Emilin-1 through the interaction with the α4β1 integrin via the C-terminal gC1q domain exerts a negative effect on proliferation. It binds pro-TGFβ preventing its maturation to mature TGFβ in the extracellular space, therefore influencing the regulation of blood and lymph vessels formation and maintenance.

Gene ID: 11117

NCBI Accession: NM_007046, NP_008977

UniProt: Q9Y6C2