MCM3 antibody

Catalog No. ABIN7539779

The Mouse Monoclonal anti-MCM3 antibody (Clone 1D4A3) (ABIN7539779) specifically detects MCM3 in WB, IHC, ICC and FACS. The antibody is reactive with Human, Rat and Monkey samples.

Quick Overview for MCM3 antibody (ABIN7539779)

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Reactivity: Human, Rat, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This MCM3 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Flow Cytometry (FACS)

Clone: 1D4A3

Product Details anti-MCM3 Antibody

Purpose: MCM3 Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human MCM3 expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes:

FCM: 1/200 - 1/400

ICC: 1/50 - 1/200

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for MCM3

Target: MCM3 (Minichromosome Maintenance Complex Component 3 (MCM3))

Alternative Name: MCM3

Background: The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Several transcript variants encoding different isoforms have been found for this gene.

Molecular Weight: 91 kDa

Gene ID: 4172

UniProt: P25205

Pathways: DNA Damage Repair, Mitotic G1-G1/S Phases, DNA Replication, Chromatin Binding, Synthesis of DNA