Acetylcholinesterase antibody

Catalog No. ABIN7539781

The Mouse Monoclonal anti-Acetylcholinesterase antibody has been validated for WB, IHC and FACS. It is suitable to detect Acetylcholinesterase in samples from Human, Rat, Mouse and Monkey.

Quick Overview for Acetylcholinesterase antibody (ABIN7539781)

Target: Acetylcholinesterase (AChE)

Reactivity: Human, Rat, Mouse, Monkey

Host: Mouse

Clonality: Monoclonal

Conjugate: This Acetylcholinesterase antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Flow Cytometry (FACS)

Clone: 3D5E8

Product Details anti-Acetylcholinesterase Antibody

Purpose: ACHE Antibody

Purification: Purified antibody

Immunogen: Purified recombinant fragment of human ACHE expressed in E. Coli.

Isotype: IgG1

Application Details

Application Notes: 1/200 - 1/400

Restrictions: For Research Use only

Handling

Buffer: Purified antibody in PBS with 0.05 % sodium azide.

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store at 4°C short term. Aliquot and store at -20°C long term. Avoid freeze/thaw cycles.

Target Details for Acetylcholinesterase

Target: Acetylcholinesterase (AChE)

Alternative Name: ACHE

Background: Acetylcholinesterase hydrolyzes the neurotransmitter, acetylcholine at neuromuscular junctions and brain cholinergic synapses, and thus terminates signal transmission. It is also found on the red blood cell membranes, where it constitutes the Yt blood group antigen. Acetylcholinesterase exists in multiple molecular forms which possess similar catalytic properties, but differ in their oligomeric assembly and mode of cell attachment to the cell surface. It is encoded by the single ACHE gene, and the structural diversity in the gene products arises from alternative mRNA splicing, and post-translational associations of catalytic and structural subunits. The major form of acetylcholinesterase found in brain, muscle and other tissues is the hydrophilic species, which forms disulfide-linked oligomers with collagenous, or lipid-containing structural subunits. The other, alternatively spliced form, expressed primarily in the erythroid tissues, differs at the C-terminal end, and contains a cleavable hydrophobic peptide with a GPI-anchor site. It associates with the membranes through the phosphoinositide (PI) moieties added post-translationally. AChE activity may constitute a sensitive biomarker of RBC ageing in vivo, and thus, may be of aid in understanding the effects of transfusion.

Molecular Weight: 67.8 kDa

Gene ID: 43

UniProt: P22303

Pathways: Skeletal Muscle Fiber Development