Calnexin antibody

Catalog No. ABIN7540476

Supplier Product No.: 100-401-e98

The Rabbit Polyclonal anti-Calnexin antibody has been validated for WB, IHC and IP. It is suitable to detect Calnexin in samples from Dog.

Quick Overview for Calnexin antibody (ABIN7540476)

Target: Calnexin (CANX)

Reactivity: Dog

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Calnexin antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP)

Product Details anti-Calnexin Antibody

Supplier Product No.: 100-401-e98

Supplier: Rockland

Purpose: Calnexin -NT Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Calnexin -NT from Human, Mouse, Rat, Bovine, Chicken, Dog, Guinea Pig, Hamster, Pig, Monkey, Rabbit, Sheep, and Xenopus based on 100 % homology with the immunizing sequence.

Purification: Anti-Calnexin -NT Antibody was prepared from monospecific antiserum by delipidation and defibrination.

Sterility: Sterile filtered

Immunogen: Calnexin-NT Antibody was produced from whole rabbit serum prepared by repeated immunizations with a 19 residue synthetic peptide of dog calnexin.

Isotype: IgG

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Western_Blot_Dilution: 1:5000-10000

Comment:

Anti-Calnexin -NT Antibody has been tested in WB, IP, IHC and IF microscopy. Expect a band approximately ~90kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for Calnexin

Target: Calnexin (CANX)

Alternative Name: CANX

Background: CANX, CNX, IP90, P90, Calnexin, pp90,Calnexin, an abundant ~90 kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90. It consists of a large 50 kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail. Unlike its ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention, calnexin has positively charged cytosolic residues that do the same thing. Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into multi-subunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans. Calnexin has also been shown to associate with the major histocompatibility complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin.

Gene ID: 403908

NCBI Accession: NP_001003232

UniProt: P24643

Pathways: MAPK Signaling, Thyroid Hormone Synthesis