HSP70 1A antibody

Catalog No. ABIN7540482

Supplier Product No.: 200-301-f64

The Mouse Monoclonal anti-HSP70 1A antibody has been validated for WB, IHC, IP and IF. It is suitable to detect HSP70 1A in samples from Chicken.

Quick Overview for HSP70 1A antibody (ABIN7540482)

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Reactivity: Chicken

Host: Mouse

Clonality: Monoclonal

Conjugate: This HSP70 1A antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Immunofluorescence (IF)

Clone: BB70

Product Details anti-HSP70 1A Antibody

Supplier Product No.: 200-301-f64

Supplier: Rockland

Purpose: Hsp70/Hsc70 Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with Hsp70/Hsc70 from Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast sources based on 100 % homology with the immunizing sequence.

Purification: Anti-Hsp70/Hsc70 Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Hsp70/Hsc70 Antibody was produced in mice by repeated immunizations raised against chicken Hsp70/Hsp90 complex.

Isotype: IgG2a

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: User Optimized

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-Hsp70/Hsc70 Antibody is tested for WB, IP, IF, and IHC. Expect a band approximately ~72 and ~73kDa proteins corresponding to the molecular mass of inducible Hsp and Hsc on SDS PAGE immunoblots. This monoclonal antibody reacts with chicken Hsp70/Hsp90 complex. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for HSP70 1A

Target: HSP70 1A (HSPA1A) (Heat Shock 70kDa Protein 1A (HSPA1A))

Alternative Name: HSPA1A

Background: Hsp70 1, Hsp70 2, Hsp70.1, Hsp72, Hsp73, HSPA1, HSPA1A, HSPA1B, Heat shock 70 kDa protein 1A/1B, Heat shock 70 kDa protein 1/2, HSP70-1/HSP70-2,Hsp70 genes encode abundant heat-inducible 70- kDa hsps (hsp70s). In most eukaryotes hsp70 genes exist as part of a multigene family. They are found in most cellular compartments of eukaryotes including nuclei, mitochondria, chloroplasts, the endoplasmic reticulum and the cytosol, as well as in bacteria. The genes show a high degree of conservation, having at least 5O% identity. The N-terminal two thirds of hsp70s are more conserved than the C-terminal third. Hsp70 binds ATP with high affinity and possesses a weak ATPase activity which can be stimulated by binding to unfolded proteins and synthetic peptides. When hsc70 (constitutively expressed) present in mammalian cells was truncated, ATP binding activity was found to reside in an N-terminal fragment of 44 kDa which lacked peptide binding capacity. Polypeptide binding ability therefore resided within the C-terminal half. The structure of this ATP binding domain displays multiple features of nucleotide binding proteins. All hsp70s, regardless of location, bind proteins, particularly unfolded ones. The molecular chaperones of the hsp70 family recognize and bind to nascent polypeptide chains as well as partially folded intermediates of proteins preventing their aggregation and misfolding. The binding of ATP triggers a critical conformational change leading to the release of the bound substrate protein. The universal ability of hsp70s to undergo cycles of binding to and release from hydrophobic stretches of partially unfolded proteins determines their role in a great variety of vital intracellular functions such as protein synthesis, protein folding and oligomerization and protein transport.

Gene ID: 3303

NCBI Accession: NP_005336

UniProt: P0DMV8

Pathways: Regulation of Leukocyte Mediated Immunity, Positive Regulation of Immune Effector Process