DLG4 antibody (Internal Region)

Catalog No. ABIN7540489

Supplier Product No.: 200-301-g11

This anti-DLG4 antibody is a Mouse Monoclonal antibody detecting DLG4 in WB, IHC, IP and FM. Suitable for Human.

Quick Overview for DLG4 antibody (Internal Region) (ABIN7540489)

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Reactivity: Human

Host: Mouse

Clonality: Monoclonal

Conjugate: This DLG4 antibody is un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC), Immunoprecipitation (IP), Fluorescence Microscopy (FM)

Clone: S28-86

Product Details anti-DLG4 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 200-301-g11

Supplier: Rockland

Purpose: Pan-MAGUK Antibody

Cross-Reactivity (Details): A BLAST analysis was used to suggest cross-reactivity with MAGUK from Mouse, Human, Zebrafish, Xenopus, and rat based on 100 % homology with the immunizing sequence.

Purification: Anti-PAN-MAGUK Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: MAGUK Antibody was produced in mice by repeated immunizations raised against a fusion protein corresponding to an internal region of human PSD95/Sap90.

Isotype: IgG1

Application Details

Application Notes:

Immunoprecipitation_Dilution: User Optimized

Immunohistochemistry_Dilution: 0.1-1.0 μg/mL

IF_Microscopy_Dilution: 1.0-10 μg/mL

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-PAN-MAGUK Antibody is tested for use in WB, IHC and IP. Expect a band approximately ~95-110kDa on specific lysates. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: 50 % (v/v) Glycerol

Preservative: 0.09 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for DLG4

Target: DLG4 (Discs, Large Homolog 4 (Drosophila) (DLG4))

Alternative Name: DLG4

Background: SAP90/PSD-95-associated protein, SAPAP, PSD-95/SAP90-binding protein, hGKAP, Guanylate kinase-associated protein, DAP, Disks large-associated protein,Membrane associated guanylate kinases (MAGUKs) form a family of scaffolding proteins, which are often associated with cellular junctions. Their capacity to serve as platforms for organizing larger protein assemblies results from the presence of several protein-protein interaction domains. PDZ-domain containing proteins of the MAGUK family target, anchor and cluster receptors and channels to subcellular sites.

Gene ID: 1742

NCBI Accession: NP_001122299

UniProt: P78352

Pathways: Regulation of Muscle Cell Differentiation, Synaptic Membrane, Skeletal Muscle Fiber Development, Asymmetric Protein Localization, Regulation of long-term Neuronal Synaptic Plasticity