CBX1 antibody

Catalog No. ABIN7540518

Supplier Product No.: 200-401-v35

The Rabbit Polyclonal anti-CBX1 antibody has been validated for WB, ChIP and FM. It is suitable to detect CBX1 in samples from Human.

Quick Overview for CBX1 antibody (ABIN7540518)

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This CBX1 antibody is un-conjugated

Application: Western Blotting (WB), Chromatin Immunoprecipitation (ChIP), Fluorescence Microscopy (FM)

Product Details anti-CBX1 Antibody

Supplier Product No.: 200-401-v35

Supplier: Rockland

Purpose: HP1 alpha, beta, gamma Antibody

Cross-Reactivity (Details): Cross-reactivity with HP1 α, β, G from species other than human has not been determined.

Purification: Anti-HP1 alpha, beta, gamma Antibody was purified by Protein G chromatography.

Sterility: Sterile filtered

Immunogen: Anti-HP1 alpha, beta, g Antibody was produced in rabbits by repeated immunization with a full length recombinant protein of human HP1 beta. The antibody also recognizes the alpha and gamma isoforms.

Isotype: IgG

Application Details

Application Notes:

ChIP_Dilution: 4 μg/ChIP

IF_Microscopy_Dilution: 1:500

Western_Blot_Dilution: 1:1,000

Comment:

Anti-HP1 α, β, g Antibody is tested in Western blot and suitable for Chromatin Immunoprecipitation and Immunofluorescence. Specific conditions for reactivity should be optimized by the end user.  Expect a band approximately 20 kDa (bands for the alpha, beta, and gamma isoforms are slightly different) in the appropriate cell lysate or extract.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.05 % (w/v) Sodium Azide and 0.05 % ProClin 300

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for CBX1

Target: CBX1 (Chromobox Homolog 1 (CBX1))

Alternative Name: CBX1

Background: Chromobox protein homolog 1, HP1Hsbeta, Heterochromatin protein 1 homolog beta, HP1-beta, Heterochromatin protein p25, M31, Modifier 1 protein, p25 beta,HP1 alpha, beta and gamma (UniProt/Swiss-Prot entry P45973, P83916 and Q13185) are components of heterochromatin. They recognize and bind histone H3 tails methylated at 'Lys-9', leading to epigenetic repression of transcription. HP1 alpha, beta and gamma also interact with lamin B receptor (LBR), thereby contributing to the association of heterochromatin with the innernuclear membrane. Anti-HP1 α, β, g Antibody is ideal for research in Chromatin Remodeling and Epigenetics.

Gene ID: 10951

NCBI Accession: NP_001120700

UniProt: P83916