5-Carboxylcytosine antibody

Catalog No. ABIN7540519

Supplier Product No.: 200-401-x50

The Rabbit Polyclonal anti-5-Carboxylcytosine antibody (ABIN7540519) specifically detects 5-Carboxylcytosine in DB, IF, IP and MA.

Quick Overview for 5-Carboxylcytosine antibody (ABIN7540519)

Target: 5-Carboxylcytosine

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Dot Blot (DB), Immunofluorescence (IF), Immunoprecipitation (IP), Multiplex Assay (MA)

Product Details

Supplier Product No.: 200-401-x50

Supplier: Rockland

Purpose: 5-caC Antibody

Cross-Reactivity (Details): Cross reactivity with other species was not tested but a wide range is presumed.

Purification: Anti-5caC Antibody was purified by Protein A purification.

Sterility: Sterile filtered

Immunogen: Anti-5caC Antibody was produced in rabbits by repeated immunizations with 5-carboxylcytosine (5caC).

Isotype: IgG

Application Details

Application Notes: Optional[Neutralization_Dilution]: Dot Blot: 1:500 - 1:1,000

Comment:

Anti-5caC Antibody is tested for Dot Blots, Immunofluorescence and Immunoprecipitation. Specific conditions for reactivity should be optimized by the end user.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M Sodium Phosphate, 0.25 M Sodium Chloride, pH 7.2

Stabilizer: 30 % Glycerol

Preservative: 0.05 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: 5-Carboxylcytosine

Target Type: Chemical

Background: 5ca-CMP monophosphate,Until recently, 5-methylcytosine (5-mC) was the only known modification of DNA for epigenetic regulation. In 2009, however, a second methylated cytosine, 5-hydroxymethylcytosine (5-hmC) was discovered. This new modified base (also called the Sixth base) is generated by enzymatic conversion of 5-mC into 5-hmC by the TET family of oxygenases. Recent results indicate that 5-hmC plays important roles distinct from 5-mC. Although its precise role has still to be shown, early evidence suggests that 5-hmC may well represent a new pathway to demethylate DNA involving a repair mechanism converting 5-hmC to cytosine. This pathway could involve further oxidation of the hydroxymethyl group to a formyl or carboxyl group followed by either deformylation or decarboxylation. The carboxyl and formyl groups of 5-Formylcytosine (5-fC) and 5-Carboxylcytosine (5-caC) could be enzymatically removed without excision of the base. Due to their structural similarity, the different modified cytosine analogues are difficult to discriminate. The development of highly specific affinity-based reagents, such as antibodies, appears to be the most powerful way to differentially and specifically enrich 5-mC and 5-hmC sequences. Anti-5caC Antibody is ideal for research in Genetics and Epigenetics.