Menin antibody (C-Term)

Catalog No. ABIN7540527

Supplier Product No.: 200-901-mj8s

The Chicken Polyclonal anti-Menin antibody has been validated for WB, ELISA and IHC. It is suitable to detect Menin in samples from Human.

Quick Overview for Menin antibody (C-Term) (ABIN7540527)

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Reactivity: Human

Host: Chicken

Clonality: Polyclonal

Conjugate: This Menin antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-Menin Antibody

Binding Specificity: C-Term

Supplier Product No.: 200-901-mj8s

Supplier: Rockland

Purpose: Anti-MEN1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Menin1.

Purification: This product is an IgY fraction antibody purified from monospecific chicken egg yolks by a multi-step process which includes selective precipitation and salt fractionation followed by extensive dialysis against the buffer stated above.

Sterility: Sterile filtered

Immunogen: Anti-Menin1 antibody was prepared from eggs of chickens laid after repeated immunizations with a synthetic peptide corresponding to a C-Terminal portion of human MEN1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-MEN1 Antibody has been tested in Western Blot and Immunohistochemistry.  Expect a band at ~67.5, 68kDa in western blot using appropriate tissues and lysates.  Anti-Menin1 should only be used for overexpressing conditions in western blot. Positive control used: Human Colon in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.002 M Sodium Phosphate, 0.15 M Sodium Chloride, pH 7.0

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Menin

Target: Menin (MEN1) (Multiple Endocrine Neoplasia 1 (MEN1))

Alternative Name: MEN1

Background: Chicken Anti-Menin1 Antibody, Menin 1, Menin, SCG2, Multiple Endocrine Neoplasia I, MEAI,Menin is a scaffold protein that functions in histone modification and epigenetic gene regulation. It is thought to regulate several pathways and processes by altering chromatin structure through the modification of histones. Essential component of a MLL/SET1 histone methyltransferase (HMT) complex, a complex that specifically methylates 'Lys-4' of histone H3 (H3K4). Functions as a transcriptional regulator. Binds to the TERT promoter and represses telomerase expression. Plays a role in TGFB1-mediated inhibition of cell-proliferation, possibly regulating SMAD3 transcriptional activity. Represses JUND-mediated transcriptional activation on AP1 sites, as well as that mediated by NFKB subunit RELA. Positively regulates HOXC8 and HOXC6 gene expression. May be involved in normal hematopoiesis through the activation of HOXA9 expression. May be involved in DNA repair. Anti-MEN1 Antibody is useful for researchers interested in Multiple Endocrine Neoplasia Type I, Insulinoma, Cancer Research and Epigenetic Research.

Gene ID: 4221

NCBI Accession: NP_000235

UniProt: O00255

Pathways: Chromatin Binding, Positive Regulation of Endopeptidase Activity