EIF2A antibody (N-Term)

Catalog No. ABIN7540569

Supplier Product No.: 600-101-ml7

The Goat Polyclonal anti-EIF2A antibody has been validated for WB, ELISA, IF and FM. It is suitable to detect EIF2A in samples from Human.

Quick Overview for EIF2A antibody (N-Term) (ABIN7540569)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-EIF2A Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-101-ml7

Supplier: Rockland

Purpose: EIF2A Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EIF2A.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-EIF2A Antibody has been tested in ELISA, WB, and IF.  Expect a band at ~65kDa in western blot using appropriate tissues and lysates.  Positive control used: HeLa in WB and IF.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background: Goat Anti-Eukaryotic Translation Initiation Factor 2A Antibody, Eukaryotic Translation Initiation Factor 2A 65 kDa, 65 KDa Eukaryotic Translation Initiation Factor 2A, EIF-2A, MSTP004, MSTP089, MST089, CDA02,EIF2A (Eukaryotic Translation Initiation Factor 2A) functions in the early steps of protein synthesis of a small number of specific mRNAs. It acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. Not be confused with eIF2-alpha (EIF2S1), the alpha subunit of the eIF2 translation initiation complex. The eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner, whereas in contrast, EIF2A binds methionyl-tRNAi to 40S subunits in a codon-dependent manner. Goat Anti-EIF2A Antibody is useful for researchers interested in phototoxic stress, translation initiation factor activity, and ribosome binding.

Gene ID: 83939

NCBI Accession: NP_001305972

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly