EIF2A antibody (N-Term)

Catalog No. ABIN7540570

Supplier Product No.: 600-101-ml7s

This anti-EIF2A antibody is a Goat Polyclonal antibody detecting EIF2A in WB, ELISA, IF and FM. Suitable for Human.

Quick Overview for EIF2A antibody (N-Term) (ABIN7540570)

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Reactivity: Human

Host: Goat

Clonality: Polyclonal

Conjugate: This EIF2A antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-EIF2A Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-101-ml7s

Supplier: Rockland

Purpose: EIF2A Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human EIF2A.

Purification: The product was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-EIF2A antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human EIF2A conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-EIF2A Antibody has been tested in ELISA, WB, and IF.  Expect a band at ~65kDa in western blot using appropriate tissues and lysates.  Positive control used: HeLa in WB and IF.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for EIF2A

Target: EIF2A (Eukaryotic Translation Initiation Factor 2A, 65kDa (EIF2A))

Alternative Name: EIF2A

Background: Goat Anti-Eukaryotic Translation Initiation Factor 2A Antibody, Eukaryotic Translation Initiation Factor 2A 65 kDa, 65 KDa Eukaryotic Translation Initiation Factor 2A, EIF-2A, MSTP004, MSTP089, MST089, CDA02,EIF2A (Eukaryotic Translation Initiation Factor 2A) functions in the early steps of protein synthesis of a small number of specific mRNAs. It acts by directing the binding of methionyl-tRNAi to 40S ribosomal subunits. Not be confused with eIF2-alpha (EIF2S1), the alpha subunit of the eIF2 translation initiation complex. The eIF-2 complex binds methionyl-tRNAi to 40S subunits in a GTP-dependent manner, whereas in contrast, EIF2A binds methionyl-tRNAi to 40S subunits in a codon-dependent manner. Goat Anti-EIF2A Antibody is useful for researchers interested in phototoxic stress, translation initiation factor activity, and ribosome binding.

Gene ID: 83939

NCBI Accession: NP_001305972

UniProt: Q9BY44

Pathways: Ribonucleoprotein Complex Subunit Organization, ER-Nucleus Signaling, Hepatitis C, Methionine Biosynthetic Process, Ribosome Assembly