MAPK1/2 (pThr202), (pTyr204) antibody

Catalog No. ABIN7540582

Supplier Product No.: 600-401-c76

The Rabbit Polyclonal anti-MAPK1/2 antibody (ABIN7540582) specifically detects MAPK1/2 in WB and IHC. The antibody is reactive with Rat samples.

Quick Overview for MAPK1/2 (pThr202), (pTyr204) antibody (ABIN7540582)

Target: MAPK1/2

Reactivity: Rat

Host: Rabbit

Clonality: Polyclonal

Conjugate: Un-conjugated

Application: Western Blotting (WB), Immunohistochemistry (IHC)

Product Details

Binding Specificity: pThr202, pTyr204

Supplier Product No.: 600-401-c76

Supplier: Rockland

Purpose: ERK-MAPK phospho T202/phospho Y204 Antibody

Cross-Reactivity (Details): ERK/MAPK pT202/pY204 antibody is directed against human ERK/MAPK.

Purification: ERK/MAPK pT202/pY204 antibodies are affinity purified from monospecific antiserum by immunoaffinity purification.

Immunogen: Anti-ERK/MAPK Antibody was produced in rabbit by repeated immunizations with synthetic phospho-peptide corresponding to amino acid residues surrounding Thr202/Tyr204 conjugated to KLH.

Isotype: IgG

Application Details

Application Notes:

Immunohistochemistry_Dilution: 1:500

Western_Blot_Dilution: 1:1000

Comment:

Anti-ERK/MAPK pT202/pY204 (Rabbit) Antibody is tested for use in Western Blotting and IHC. Specific conditions for reactivity should be optimized by the end user. Expect a band of approximately 42-44 kDa in size corresponding to ERK/MAPK phosphorylated at Thr202 and Tyr204.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.01 M HEPES, 0.15 M Sodium Chloride, pH 7.5

Stabilizer: 0.1 mg/mL Bovine Serum Albumin (BSA) - IgG and Protease free, 50 % (v/v) Glycerol

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. This product is stable at 4° C as an undiluted liquid. For extended storage, aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details

Target: MAPK1/2

Background: Mitogen-activated protein kinase 1, MAP kinase 1, MAPK1, ERT1, Extracellular signal-regulated kinase 2, MAP kinase isoform p42,Anti-ERK/MAPK pT202/pY204 Antibody detects ERK/MAPK which is an integral component of cellular signaling during mitogenesis and differentiation of mitotic cells and also is thought to play a key role in learning and memory. The activity of this kinase is regulated by dual phosphorylation at Thr202 and Tyr204. Anti-ERK/MAPK pT202/pY204 Antibody is ideal for investigators involved in Cell Signaling and Cancer Research.

Gene ID: 116590

NCBI Accession: NP_446294

UniProt: P63086