HEY1 antibody (Internal Region)

Catalog No. ABIN7540592

Supplier Product No.: 600-401-hb5s

This Rabbit Polyclonal antibody specifically detects HEY1 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for HEY1 antibody (Internal Region) (ABIN7540592)

Target: HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This HEY1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-HEY1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-hb5s

Supplier: Rockland

Purpose: HEY1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human HEY1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-HEY1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human HEY1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1:1000

Comment:

Anti-HEY1 Antibody has been tested in Western Blot, ELISA, and Immunohistochemistry. Expect a band at ~33 kDa in western blot using appropriate tissues or lysates.  Positive control used is a recombinant human Hey1 at 1μg/mL for WB, human cerebellum tissue at 1:100 for IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for HEY1

Target: HEY1 (Ha-Ry/enhancer-of-Split Related with YRPW Motif 1 (HEY1))

Alternative Name: HEY1

Background: Rabbit Anti-HEY1 Antibody, BHLHB31, CHF2, HERP2, HESR1, HRT1, Hairy/Enhancer-Of-Split Related With YRPW Motif 1, Hes Related Family BHLH Transcription Factor With YRPW Motif 1, Hairy And Enhancer Of Split-Related Protein 1, Class B Basic Helix-Loop-Helix Protein 31, Cardiovascular Helix-Loop-Helix Factor 2, Hairy-Related Transcription Factor 1, HES-Related Repressor Protein 1, HRT-1, HESR-1, CHF-2, OAF1,HEY1 belongs to the hairy and enhancer of split-related family (HESR), a transcriptional repressor family.  This nuclear protein is induced by the Notch pathway and is mainly associated with cardiovascular development. This family is known to repress GATA4, GATA6, MASH1, and NEUROD4 downstream effects. It's role in liver cancer cell self-renewal is still being studied, as it's suspected role in neurogenesis in mice. Hey1 is associated with diseases such as mesenchymal and periosteal chondrosarcoma. Anti-HEY1 antibody is useful for researchers interested in cancer research, neuroscience research, and Notch signalling.

Gene ID: 23462

NCBI Accession: NP_001035798

UniProt: Q9Y5J3

Pathways: Tube Formation