ERN1 antibody (Internal Region)

Catalog No. ABIN7540596

Supplier Product No.: 600-401-hb9s

The Rabbit Polyclonal anti-ERN1 antibody has been validated for WB, ELISA, IHC, IF and FM. It is suitable to detect ERN1 in samples from Human.

Quick Overview for ERN1 antibody (Internal Region) (ABIN7540596)

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This ERN1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-ERN1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-hb9s

Supplier: Rockland

Purpose: Ire1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Ire1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Ire1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Ire1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000-1:50,000

Immunohistochemistry_Dilution: 1:100

IF_Microscopy_Dilution: 10 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-Ire1 Antibody has been tested in ELISA, Western Blot, Immunofluorescence, and Immunohistochemistry. Expect a band in mouse for isoform 1 at ~110.2kDa and 2 at ~45.3kDa, and in human at ~109.7 kDa in western blot using appropriate lysates or tissues.  Positive control used: Mouse brain tissue in Western Blot,  SCLC-21H cells in IF, and Human stomach tissue in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for ERN1

Target: ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1 (ERN1))

Alternative Name: Ern1

Background: Rabbit Anti-Ern1 Antibody, Rabbit Anti-Endoplasmic Reticulum To Nucleus Signaling 1 Antibody, Rabbit Anti-IRE1 Antibody, Endoplasmic Reticulum To Nucleus Signaling 1, Serine/Threonine-Protein Kinase/Endoribonuclease IRE1, Inositol-Requiring Protein 1, Inositol-Requiring Enzyme 1, ER To Nucleus Signalling 1, Ire1-Alpha, HIRE1p, IRE1a, IRE1, Endoplasmic Reticulum-To-Nucleus Signaling 1, Protein Kinase/Endoribonuclease, Inositol-Requiring 1, IRE1P,Inositol-requiring enzyme 1 (IRE1) or ERN1 (Endoplasmic Reticulum To Nucleus Signaling 1) is a Serine/threonine-protein kinase and endoribonuclease that acts as a key sensor for the endoplasmic reticulum unfolded protein response (UPR). In unstressed cells, the endoplasmic reticulum luminal domain is maintained in its inactive monomeric state by binding to the endoplasmic reticulum chaperone HSPA5/BiP. Accumulation of misfolded protein in the endoplasmic reticulum causes release of HSPA5/BiP, allowing the luminal domain to homodimerize, promoting autophosphorylation of the kinase domain, and subsequent activation of the endoribonuclease activity. The endoribonuclease activity is specific for XBP1 mRNA and excises 26 nucleotides from XBP1 mRNA. The resulting spliced transcript of XBP1 encodes a transcriptional activator protein that up-regulates expression of UPR target genes. Diseases associated with ERN1 include Chromosome 6Pter-P24 Deletion Syndrome and Alzheimer Disease. Anti-IRE1 Antibody is useful for researchers interested in Neuroscience research.

Gene ID: 78943

NCBI Accession: NP_076402

UniProt: Q9EQY0

Pathways: ER-Nucleus Signaling, Unfolded Protein Response