SMO antibody (Internal Region)

Catalog No. ABIN7540600

Supplier Product No.: 600-401-mg0s

This anti-SMO antibody is a Rabbit Polyclonal antibody detecting SMO in WB, ELISA and IHC. Suitable for Human.

Quick Overview for SMO antibody (Internal Region) (ABIN7540600)

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMO antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-SMO Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mg0s

Supplier: Rockland

Purpose: Smoothened Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SMO.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Smoothened antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Smoothened conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Western_Blot_Dilution: 1 μg/mL

Comment:

Anti-Smoothened Antibody has been tested in ELISA, WB, and IHC.  Expect a band at ~86.4kDa in western blot using appropriate lysates and tissues.  Positive control used: Overexpressing lysate and HEK293T in Western Blot, and Human Lung Tissue in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SMO

Target: SMO (Smoothened Homolog (Drosophila) (SMO))

Alternative Name: SMO

Background: Rabbit Anti-SMO Antibody, Smoothened Frizzled Class Receptor,  Smoothened Seven Transmembrane Spanning Receptor, Smoothened Frizzled Family Receptor, Frizzled Family Member 11, Protein GX, SMOH, Seven Transmembrane Helix Receptor, Smoothened (Drosophila) Homolog, Smoothened Homolog (Drosophila), Smoothened Homolog, FZD11, CRJS, Gx,SMO (Smoothened, Frizzled Class Receptor) receptors are non-classical G protein-coupled receptors that probably associates with the patched protein (PTCH) to transduce the hedgehogs proteins signal. The binding of sonic hedgehog (SHH) to its receptor patched is thought to prevent normal inhibition by patched of smoothened (SMO). It is required for the accumulation of KIF7, GLI2 and GLI3 in the cilia. And it interacts with DLG5 at the ciliary base to induce the accumulation of KIF7 and GLI2 at the ciliary tip for GLI2 activation.  Diseases associated with SMO include Curry-Jones Syndrome and Basal Cell Carcinoma.  Anti-Smoothened Antibody is useful for researchers interested in Cancer research.

Gene ID: 6608

UniProt: Q99835

Pathways: Hedgehog Signaling