Beclin 1 antibody (N-Term)

Catalog No. ABIN7540604

Supplier Product No.: 600-401-mg4s

This Rabbit Polyclonal antibody specifically detects Beclin 1 in WB, ELISA, IHC, IF and FM. It exhibits reactivity toward Human.

Quick Overview for Beclin 1 antibody (N-Term) (ABIN7540604)

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This Beclin 1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-Beclin 1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-mg4s

Supplier: Rockland

Purpose: Beclin 1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human Beclin-1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-BECLIN1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a near N-terminal portion of human Beclin-1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100-1:200

IF_Microscopy_Dilution: 5 μg/mL

Western_Blot_Dilution: 1:1000

Comment:

Anti-BECLIN-1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~51.8kDa in western blot using appropriate lysates.  Positive control used: U251-MG in WB,  HeLa in IF, Human spleen and colon cancer tissues in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for Beclin 1

Target: Beclin 1 (BECN1) (Beclin 1, Autophagy Related (BECN1))

Alternative Name: BECN1

Background: Rabbit Anti-Beclin-1, Beclin1 Antibody, Beclin 1 Antibody, BECN1, Coiled-Coil Myosin-Like BCL2-Interacting Protein, Beclin 1 Autophagy Related, Beclin 1 (Coiled-Coil, Moesin-Like BCL2 Interacting Protein), ATG6 Autophagy Related 6 Homolog, Protein GT197, VPS30, GT197,BECLIN1 belongs to the beclin family. Beclin 1 plays a central role in autophagy, a catabolic process of degradation induced by starvation. Acts as core subunit of the PI3K complex that mediates formation of phosphatidylinositol 3-phosphate which mediates vesicle-trafficking processes. This protein is thought to play a role in multiple cellular processes, including tumorigenesis, neurodegeneration and apoptosis. May play a role in antiviral host defense.  Protects against infection by a neurovirulent strain of Sindbis virus. Localized in the Golgi apparatus > trans-Golgi network membrane. Interaction with ATG14 promotes translocation to autophagosomes. Expressed in dendrites and cell bodies of cerebellar Purkinje cells.  Anti-Beclin-1 Antibody is useful for researchers interested in Cancer Research including Cervix Carcinoma and Breast Cancer. 

Gene ID: 8678

NCBI Accession: NP_001300927

UniProt: Q14457

Pathways: Autophagy