MECP2 antibody (C-Term)

Catalog No. ABIN7540619

Supplier Product No.: 600-401-mm0

This Rabbit Polyclonal antibody specifically detects MECP2 in WB, ELISA and IHC. It exhibits reactivity toward Human.

Quick Overview for MECP2 antibody (C-Term) (ABIN7540619)

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This MECP2 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC)

Product Details anti-MECP2 Antibody

Binding Specificity: C-Term

Supplier Product No.: 600-401-mm0

Supplier: Rockland

Purpose: MeCP2 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human MeCP2.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-MeCP2 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to C-terminal portion of human MeCP2 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:15,000

Immunohistochemistry_Dilution: 1:200

Western_Blot_Dilution: 1:1000

Comment:

Suggested Applications: This antibody is not suitable for CHIP.
Anti-MeCP2 Antibody has been tested in ELISA, Western Blot, and Immunohistochemistry. Expect a band at ~52 kDa in western blot using appropriate lysates or tissues. Positive control used U2-OS, A431, human cerebellum tissue, and Chicken Spinal Cord in WB, human tonsil tissue in IHC.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for MECP2

Target: MECP2 (Methyl CpG Binding Protein 2 (MECP2))

Alternative Name: MECP2

Background: Rabbit Anti-Methyl-CpG Binding Protein 2 Antibody, Rabbit-Anti-MeCP2 Antibody, MeCp-2 Protein, Methyl CpG Binding Protein 2 (Rett Syndrome), Testis Tissue Sperm-Binding Protein Li 41a, Mental Retardation, X-Linked 16, Mental Retardation, X-Linked 79, Methyl-CpG-Binding Protein 2, Methyl CpG Binding Protein 2, Rett Syndrome, AUTSX3, MRXS13, MRXSL, MRX16, MRX79, MeCp2, PPMX, RTS, RTT, RS,MeCP2 (Methyl-CpG Binding Protein 2) is a chromosomal protein that binds to methylated DNA. DNA methylation is the major modification of eukaryotic genomes and plays an essential role in mammalian development. Human proteins MECP2, MBD1, MBD2, MBD3, and MBD4 comprise a family of nuclear proteins related by the presence in each of a methyl-CpG binding domain (MBD). It can bind specifically to a single methyl-CpG pair. It is not influenced by sequences flanking the methyl-CpGs. MECP2 mediates transcriptional repression through interaction with histone deacetylase and the corepressor SIN3A. It binds both 5-methylcytosine (5mC) and 5-hydroxymethylcytosine (5hmC)-containing DNA, with a preference for 5-methylcytosine (5mC). Anti-MeCP2 Antibody is useful for researchers interested in Rett Syndromes, SIDS, Mental Retardation X-Linked Syndromic 13, and chromatin binding pathways.

Gene ID: 4204

NCBI Accession: NP_001104262

UniProt: P51608

Pathways: Inositol Metabolic Process, Chromatin Binding, Synaptic Membrane