SREBF1 antibody (N-Term)

Catalog No. ABIN7540627

Supplier Product No.: 600-401-mn1

This anti-SREBF1 antibody is a Rabbit Polyclonal antibody detecting SREBF1 in WB and ELISA. Suitable for Human.

Quick Overview for SREBF1 antibody (N-Term) (ABIN7540627)

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SREBF1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA

Product Details anti-SREBF1 Antibody

Binding Specificity: N-Term

Supplier Product No.: 600-401-mn1

Supplier: Rockland

Purpose: SREBP1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human SREBP1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-SREBP1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-terminal portion of human SREBP1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-SREBP1 Antibody has been tested in ELISA and WB.  Expect reactivity with different human isoforms and a band at ~124.6, ~121.7, ~113.6, ~48.3kDa in western blot using appropriate tissue or lysates.  Positive control used: HeLa and A549 lysates in Western Blot.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for SREBF1

Target: SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1 (SREBF1))

Alternative Name: SREBF1

Background: Rabbit Anti-SREBP1 Antibody, Rabbit Anti-Sterol Regulatory Element Binding Transcription Factor 1 Antibody, Class D Basic Helix-Loop-Helix Protein 1, SREBP1, BHLHd1, Sterol Regulatory Element-Binding Transcription Factor 1, Sterol Regulatory Element-Binding Protein 1, SREBP-1, BHLHD1,SREBF1 (Sterol Regulatory Element Binding Transcription Factor 1) is a transcriptional activator required for lipid homeostasis. It regulates transcription of the LDL receptor gene as well as the fatty acid and to a lesser degree the cholesterol synthesis pathway. It binds to the sterol regulatory element 1 (SRE-1) (5-ATCACCCCAC-3), which is a motif that is found in the promoter of the low-density lipoprotein receptor gene and other genes involved in sterol biosynthesis. SREBP1 has dual sequence specificity binding to both an E-box motif (5-ATCACGTGA-3) and to SRE-1 (5-ATCACCCCAC-3). Anti-SREBP1 Antibody is useful for researchers interested in Fatty Liver Disease, Smith-Magenis Syndrome, Adipogenesis, DNA binding transcription factor activity and chromatin binding.

Gene ID: 6720

NCBI Accession: NP_001005291

UniProt: P36956

Pathways: AMPK Signaling, Caspase Cascade in Apoptosis, Negative Regulation of Hormone Secretion, Regulation of Lipid Metabolism by PPARalpha