LC3B antibody (Internal Region)

Catalog No. ABIN7540630

Supplier Product No.: 600-401-mn5

This anti-LC3B antibody is a Rabbit Polyclonal antibody detecting LC3B in WB, ELISA, IHC and FACS. Suitable for Human.

Quick Overview for LC3B antibody (Internal Region) (ABIN7540630)

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Reactivity: Human

Host: Rabbit

Clonality: Polyclonal

Conjugate: This LC3B antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Flow Cytometry (FACS)

Product Details anti-LC3B Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mn5

Supplier: Rockland

Purpose: LC3B Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against human LC3B.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-LC3B antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of human Microtubule-associated proteins 1A/1B light chain 3B conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 1:10,000 - 1:50,000

Immunohistochemistry_Dilution: 1:100

Flow_Cytometry_Dilution: 1:25

Western_Blot_Dilution: 1:1000

Comment:

Anti-LC3B Antibody has been tested in ELISA, Western Blot, Immunohistochemistry, and Flow Cytometry. Positive control used U251-MG Cells in Flow and Human lymph node in IHC. Testing only recommended in WB when using recombinant or overexpressing systems. Expect a band at ~14.6 kDa in western blot using appropriate lysates or tissues.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: 4 °C,-20 °C

Storage Comment: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.

Expiry Date: 12 months

Target Details for LC3B

Target: LC3B (MAP1LC3B) (Microtubule-Associated Protein 1 Light Chain 3 beta (MAP1LC3B))

Alternative Name: MAP1LC3B

Background: Rabbit Anti-Microtubule Associated Protein 1 Light Chain 3 Beta Antibody, Rabbit Anti-LC3B Antibody, Microtubule-Associated Proteins 1A/1B Light Chain 3B, Autophagy-Related Ubiquitin-Like Modifier LC3 B, MAP1 Light Chain 3-Like Protein 2, MAP1A/MAP1B Light Chain 3 B, MAP1A/MAP1B LC3 B, Microtubule-Associated Protein 1 Light Chain 3 Beta, Autophagy-Related Protein LC3 B, MAP1A/1BLC3, MAP1LC3B-A, MAP1ALC3, ATG8F, LC3B,MAP1LC3B (Microtubule Associated Protein 1 Light Chain 3 Beta) is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. It is a ubiquitin-like modifier involved in formation of autophagosomal vacuoles (autophagosomes). LC3B plays a role in mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Whereas LC3s are involved in elongation of the phagophore membrane, the GABARAP/GATE-16 subfamily is essential for a later stage in autophagosome maturation. Promotes primary ciliogenesis by removing OFD1 from centriolar satellites via the autophagic pathway. Anti-LC3B Antibody is useful for researchers interested in spastic paraparesis, neuroendocrine system, neuroscience research, cancer research and apoptosis research.

Gene ID: 81631

NCBI Accession: NP_073729

UniProt: Q9GZQ8

Pathways: Autophagy