SMAD1 antibody (Internal Region)

Catalog No. ABIN7540633

Supplier Product No.: 600-401-mn6s

This anti-SMAD1 antibody is a Rabbit Polyclonal antibody detecting SMAD1 in WB, ELISA, IHC, IF and FM. Suitable for Mouse.

Quick Overview for SMAD1 antibody (Internal Region) (ABIN7540633)

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Reactivity: Mouse

Host: Rabbit

Clonality: Polyclonal

Conjugate: This SMAD1 antibody is un-conjugated

Application: Western Blotting (WB), ELISA, Immunohistochemistry (IHC), Immunofluorescence (IF), Fluorescence Microscopy (FM)

Product Details anti-SMAD1 Antibody

Binding Specificity: Internal Region

Supplier Product No.: 600-401-mn6s

Supplier: Rockland

Purpose: Smad1 Antibody

Cross-Reactivity (Details): This affinity purified antibody is directed against mouse Smad1.

Purification: The antibody was affinity purified from monospecific antiserum by immunoaffinity purification.

Sterility: Sterile filtered

Immunogen: Anti-Smad1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to an internal portion of mouse Smad1 conjugated to Keyhole Limpet Hemocyanin (KLH).

Isotype: IgG

Application Details

Application Notes:

ELISA_Dilution: 5 μg/mL

Immunohistochemistry_Dilution: 1:400-1:500

IF_Microscopy_Dilution: 5-10 μg/mL

Western_Blot_Dilution: 5 μg/mL

Comment:

Anti-Smad1 Antibody has been tested in ELISA, WB, IF, and IHC.  Expect a band at ~52.2kDa in western blot using appropriate tissues or lysates.  Positive control used: Mouse testis and C2C12 in Western Blot, C2C12 in Immunofluorescence, Mouse Lung, Skeletal Muscle, and Testis in Immunohistochemistry.

Restrictions: For Research Use only

Handling

Format: Liquid

Buffer:

Buffer: 0.02 M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2

Stabilizer: None

Preservative: 0.01 % (w/v) Sodium Azide

Preservative: Sodium azide

Precaution of Use: This product contains Sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Storage: -20 °C

Storage Comment: Store vial at -20° C or below prior to opening. This vial contains a relatively low volume of reagent (25 μL). To minimize loss of volume dilute 1:10 by adding 225 μL of the buffer stated above directly to the vial. Recap, mix thoroughly and briefly centrifuge to collect the volume at the bottom of the vial. Use this intermediate dilution when calculating final dilutions as recommended below. Store the vial at -20°C or below after dilution. Avoid cycles of freezing and thawing.

Expiry Date: 12 months

Target Details for SMAD1

Target: SMAD1 (SMAD, Mothers Against DPP Homolog 1 (SMAD1))

Alternative Name: Smad1

Background: Mothers against decapentaplegic homolog 1, MAD homolog 1, Mothers against DPP homolog 1, Dwarfin-A, Dwf-A, Mothers-against-DPP-related 1, Mad-related protein 1, mMad1, SMAD 1, Smad1, Madh1, Madr1,Smad1 is also known Mothers Against Decapentaplegic homolog 1, Mothers against DPP homolog 1, hSMAD-3, JV4-1, Transforming growth factor-Beta-Signaling Protein 1 or BSP1. SMAD proteins are signal transducers and transcriptional modulators that mediate multiple signaling pathways. SMAD1, as a transcriptional modulator, is activated by BMP (Bone Morphogenetic Protein) type 1 receptor kinase (it is a receptor-regulated SMAD or R-SMAD). BMPs are involved in a range of biological activities including cell growth, apoptosis, morphogenesis, development and immune responses. SMAD proteins have been implicated as downstream effectors of TGF beta/BMP signaling. In response to BMP ligands, SMAD1 can be phosphorylated (other sites besides the most prominent of S206, are S187, S195, and S214). S-206 is phosphorylated by ERK in response to mitogenic growth factors, or by recombinant ERK in vitro, this can be tested by treating cells with EGF or in cancer cells where Ras is activated. The phosphorylated form of this protein forms a complex with SMAD4, which is important for its function in the transcription regulation. This protein is also a target for SMAD-specific E3 ubiquitin ligases, such as SMURF1 and SMURF2, and undergoes ubiquitination and proteasome-mediated degradation.

Gene ID: 17125

NCBI Accession: NP_032565

UniProt: P70340

Pathways: Stem Cell Maintenance, Regulation of Muscle Cell Differentiation, Skeletal Muscle Fiber Development